【摘要】 目的:研究复方地黄2种醇提取物对链脲佐菌素(STZ)诱导糖尿病大鼠的早期视网膜病变及糖尿病肾病的干预作用。方法:腹腔注射STZ 60 mg.kg-1建立糖尿病大鼠模型,4周后随机分组,5~8周3个治疗组分别每日给予氨基胍(100 mg.kg-1),复方地黄70%乙醇提取物(10 g.kg-1)及复方地黄95%乙醇提取物(10 g.kg-1)。8周后测定各组动物血清中血糖(G lu)、肌酐(Cr)和尿素氮(BUN)的含量,并测定肾组织中过氧化氢酶(CAT)活性、诱导型一氧化氮合成酶(iNOS)活性、一氧化氮(NO)和羟脯氨酸(HYP)含量;用RT-PCR测定视网膜PreproET-1和MMP-9 mRNA的表达。结果:与正常对照组比较,模型组大鼠血清Cr及BUN的含量以及肾脏组织iNOS活性、NO和HYP含量均显著升高,肾脏组织CAT活性显著降低,视网膜Prepro ET-1 mRNA表达量显著升高,MMP-9 mR-NA表达量显著降低。复方地黄70%和95%乙醇提取物组的血糖虽然无明显降低,但上述异常指标均明显逆转,与阳性对照组氨基胍接近。结论:复方地黄70%和95%乙醇提取物均能改善STZ诱导的糖尿病大鼠早期视网膜病变及糖尿病肾病发生,药效与AGEs受体拮抗剂氨基胍相当。更多还原

【Abstract】 Objective: To explore the effectiveness of two ethanol extracts of compound Rehmannia Root decoction on diabetic retinopathy and nephropathy in STZ-induced diabetes rats.Methods: Rats were injected i.p.by STZ 60 mg·kg-1,and diabetes model was developed in 8 weeks.The STZ groups were treated with aminoguanidine(100 mg·kg1),70% ethanol extracts(10 g·kg-1) and 95% ethanol extracts(10 g·kg-1) from 5th week to 8th week,respectively.The laboratory indices,including blood glucose,creatinine(Cr),urea nitrogen(BUN) in serum;and catalase(CAT),inducible nitric oxide synthase(iNOS),nitrogen monoxidum(NO),hydorxyproline(HYP) in kidney,were determined after eight weeks.PreproET1 and MMP-9 mRNA expression were determined by RT-PCR in retina.Results: After 8 weeks of diabetes inducement,Cr and BUN levels in serum increased;NO,iNOS,CAT and HYP in kidney displayed significant pathological changes.mRNA expression of PreproET-1 increased,MMP-9 mRNA expression was markedly reduced.Following interventions,these changes were dramatically reversed by aminoguanidine,95% and 70% ethanol extracts.95% ethanol extract was more effective than 70% ethanol in some aspects.Conclusion: Effectiveness of ethanol extracts of compound Rehmannia decoction is comparable to those of aminoguanidine.Lipid soluble components in ethanol extract are similarly effective to attenuate complications of diabetes in rats.更多还原