【摘要】 目的:探讨富硒姬松茸提取物-硒蛋白多糖(SPP)处理的小鼠血清在体外对K562细胞凋亡的影响及机制。方法:采用血清药理学法制备SPP处理血清,MTT法观察SPP处理的小鼠血清(终浓度10%)对K562细胞增殖的影响;从形态学、DNA电泳技术观察凋亡发生,比色法检测凋亡相关基因Caspase-3的表达。结果:SPP处理的小鼠血清可显著抑制白血病K562细胞生长,且呈时间剂量依赖性。形态学和DNA电泳结果均证实有凋亡发生。与空白血清组比较,Caspase-3基因上调明显。SPP处理的荷瘤小鼠血清抗肿瘤效果明显优于正常小鼠的处理血清。结论:SPP处理的荷瘤小鼠血清可促K562细胞凋亡,其机制可能与上调Caspase-3基因有关。更多还原

【Abstract】 Objective:To explore the effect and mechanism of serum treated with selenium-protein polysaccharide(SPP),extracted from rich-selenium Agaricus blazei,inducing apoptosis of K562 cells in vitro and compare the difference of antitumor activity of serum treated with SPP between normal and pathological mice.Methods:According to serum pharmacology,serum treated with SPP was prepared.Growth inhibition rate of K562 cells was detected by MTT method.Apoptotic phenomenon was observed under a fluorescent microscope and through DNA electrophoresis,respectively.Caspase-3 enzymatic activity was measured by colorimetry.Results:Serum treated with SPP(10%)could significantly inhibit the growth of K562 cells.There was a markedly positive correlation between drug dose(50,100 mg·kg-1),time(24～48 h)and inhibitory rate.Apoptotic phenomenon was showed by morphology and DNA electrophoresis.Compared with control group,Caspase-3 gene was significantly up-regulated.Compared with the serum treated with SPP of nonbearing tumor group,that of bearing tumor group has much more potent effect on antitumor.Conclusion:Serum treated with SPP could induce apoptosis of K562 cells.Its mechanism may be related to up-regulation of Caspase-3.更多还原