【摘要】 目的评价α1受体阻断剂(特拉唑嗪)对慢性心力衰竭(CHF)大鼠心肌神经生长因子(NGF)表达水平的影响。方法选24只雄性Wistar大鼠,应用阿霉素腹腔注射的方法制作CHF模型;随机分成特拉唑嗪治疗组(DCM-T)和安慰剂治疗组(DCM-P),DCM-T组接受特拉唑嗪灌胃治疗,0.4mg/kg·d,DCM-P组用淀粉代替特拉唑嗪,疗程6周;RT-PCR和Western blot方法评价心肌NGF表达水平。结果DCM-P组大鼠心肌NGFmRNA和蛋白表达水平比正常对照组明显下降(51.7%vs45.0%,均P<0.01);DCM-T组NGFmRNA和蛋白下降值明显低于DCM-P组(35.0%vs51.7%和21.0%vs45.0%,均P<0.05)。结论CHF大鼠心肌NGF表达水平明显下降;特拉唑嗪治疗能够阻止CHF大鼠心肌NGF下调,有助于改善CHF大鼠心肌交感神经重构。更多还原

【Abstract】 [Objective] To investigate the effect of alpha 1 adrenoceptor blocker(Terazosin) on the expression of beta-nerve growth factor in myocardium of rats with heart failure.[Methods] Thirty adult male Wistar rats were randomly divided into DCM group(n =24) and normal control group(NC,n =6).Then DCM group was randomly divided into DCM-T group(n =12) and DCM-P group(n =12);Adrimycin was injected by intraperitoneal to rats of the DCM group at a dose of 2 mg/kg for 10 times.Terazosin was used to treat the heart failure at a dose of 0.4 mg/kg per day in DCM-T group,while as a placebo,starch was used to treat the heart failure at the same dose in DCM-P group;Nerve growth factor mRNA was measured with RT-PCR;Nerve growth factor protein was measured with Western Blot [Results] Compared with NC group,NGF mRNA and protein were significant decreased in DCM-P group(51.7% and 45.3%,all P <0.01);NGFmRNA and protein were less decreased in DCM-T group than in DCM-P group(35.0% vs 51.7% and 21.8% vs 45.0%,all P <0.05).[Conclusions] bNGF were significant decreased in the myocardium of heart failure rats;Terazosin prevents the decrease of bNGF in myocardium of rats with heart failure.更多还原