【摘要】 目的:建立杜仲茶中桃叶珊瑚甙含量测定的超高压提取(UHPE)-高效液相色谱(HPLC)分析法。方法:采用正交试验考察超高压提取压力、提取时间、料液比和甲醇浓度对提取桃叶珊瑚甙的影响,以HPLC法为含量测定方法。结果:最佳提取桃叶珊瑚甙的条件为超高压提取压力350 MPa、时间3 min、料液比1:8、70%甲醇。HPLC法测定桃叶珊瑚甙的线性范围在5~100μg/ml,相关系数为0.9998。杜仲茶中桃叶珊瑚甙的含量为1.94%,相对标准偏差为3.02%,加标回收率为97.40%~100.16%。结论:此法简单、快速、经济,是测定杜仲茶中桃叶珊瑚甙含量的有效方法。更多还原

【Abstract】 Objective:To establish a sensitive analytical method for detection of the aucubin in eucommia tea by using ultra high pressure extract(UHPE) and high performance liquid chromatograpy(HPLC).Methods:Orthogonal test was used to evaluate the influence of the 4 factors including the UHPE pressure,UHPE time,the ratio of solid to liquid and the extracting concentration of methanol.HPLC method was used to determine the aucubin in eucommia tea.Results:The optimum extraction was as follows: UHPE pressure 350 MPa,UHPE time 3 min,stock ratio 1:8(g:ml) and 70% methanol.The linearity was superior from 5～100 μg/ml.In eucommia tea,the content of aucubin was about 1.94%,the standard deviation was 3.02%.The average recovery rate was 97.40%～100.16%.Condution:This method is quick and economical.It has been satisfactorily applied to the aucubin analyses.更多还原