【摘要】 目的研究电磁脉冲(EMP)辐照对白血病肿瘤细胞株Jurkat细胞分泌细胞因子IFN-γ和IL-4的影响,探讨EMP对Jurkat细胞损伤机制。方法Jurkat细胞经EMP辐照后即刻、1 h、6 h、12h、24 h、48 h、72 h共7个时相点,细胞悬液甩片,4%多聚甲醛4℃固定,自然晾干,进行SP法的IFN-γ和IL-4蛋白免疫组化染色。在光镜10×40倍视野下,应用CM IAS-H图像分析仪对阳性细胞的图像学参数的积分光密度值(IOD)进行测量和统计分析。结果免疫组化结果表明,场强6×104V/m的电磁脉冲能使Jurkat细胞IFN-r表达持续性减少,差异非常显著(P<0.01),而IL-4变化不明显(P>0.05)。结论EMP辐照后Th1型的细胞因子IFN-γ明显减少,而Th2型的细胞因子IL-4未见显著变化,提示EMP辐照后机体中受到影响的主要是细胞免疫,而体液免疫功能未受到明显影响。更多还原

【Abstract】 Objective With observing the changes of expression of IFN-γ and IL-4 in Jurkat cell after being exposure to electromagnetic to explore the possible injury mechanism.Methods The Jurkat cell were collected.at.instantly,1 h,6 h,12 h,24 h,48 h,72 h respectively after exposure to EMP.The expression of IFN-γ and IL-4 in Jurkat cells were determined by immunohistochemical SP method.stereological analysis for over 100.cells on the glass slide were performed by CMTAS-Ⅱ image analysis system at a magnification 400.ALL data was analyzed by SPSS8.0 software.The significance of differences among groups was analyzed with mono-factor analysis of variance.Results IL-4 has not statistically change but IFN-r both has decreased.statistically.Conclusion cell mediated immunity maybe plays an important role in the injury of Jurkat cell by EMP.更多还原