【摘要】 目的探讨耐异烟肼结核分支杆菌KatG基因突变在结核分支杆菌耐异烟肼耐药性测定中的应用价值。方法采用聚合酶链反应——单链构象多态性(PCR-SSCP)分析72株结核分支杆菌KatG基因突变。其中32株为异烟肼(INH)敏感株,40株为INH耐药株,用PCR-SSCP图谱鉴定扩增产物有无突变,H37RV标准株作对照。结果所有INH敏感株SSCP带谱与对照相同;40株INH耐药株中15株与对照相同,25株有不同程度的差异,INH耐药KatG基因突变或缺失的阳性率为62.5%。结论多数结核分支杆菌耐INH是由于其KatG基因突变所致,用PCR-SSCP筛选突变株可达到快速检测结核分支杆菌INH耐药的目的。更多还原

【Abstract】 Objective To observe the mutations of katG in Mycobacterium tuberculosis isoniazid-resident isolates,to research their l value in isoniazid resistanceMethods Analyzing the mutations of katG in 72 Mycobacterium tuberculosis with PCR-SSCP.32 isoniazid susceptible Mycobacterium tuberculosis clinical isolates and 40 isoniazid resistant isolates with Mycobacterium tuberculosis H37RV reference strains as control group.Results Amplification was abserved all of isoniazid susceptible Mycobacterium tuberculosis clinical isolates.Among 40 isoniazid resistant chinial isolates,15 isolates diaplayed the same patterns as H37RV and 25 isolates showed PCR-SSCP diffirent from that of H37RV.About 62.5 percent isoniazid-resident isolates showed mutation or deletion of katG gene.Conclusion The changes of katG gene are associated with isoniazid residenance.PCR-SSCP technique will be useful to rapidly screen gene mutation of isoniazid resistant Mycobacterium tuberculosis.更多还原