【摘要】 在测定替米考星对鸡毒支原体、多杀巴氏杆菌和猪胸膜肺炎放线杆菌的最小抑菌浓度基础上,采用药物浓度递增法体外诱导3种病原菌对替米考星的耐药性。结果鸡毒支原体经9次传代对替米考星产生了高水平耐药,多杀巴氏杆菌经7~9次传代对替米考星产生了高水平耐药,猪胸膜肺炎放线杆菌经14次传代对替米考星的耐受浓度未发生明显变化;鸡毒支原体和多杀巴氏杆菌替米考星诱导耐药株对红霉素、阿奇霉素、泰乐菌素、吉他霉素和林可霉素表现交叉耐药。研究结果提示,替米考星较易诱导鸡毒支原体和多杀巴氏杆菌产生耐药性,兽医临床应合理应用。更多还原

【Abstract】 Induction of tilmicosin resistance in Mycoplasma gallisepticum(MG),Pasteurella multocida(PM) and Actinobacillus pleuropneumoniae(App) were performed by in vitro exposure to stepwise concentration of tilmicosin,and the MICs of macrolides and lincomycin for tilmicosin-resistant mutants were determined.High resistance to tilmicosin appeared after 9 passages in MG and after 7～9 passages in PM.But for App,the tolerated concentration of tilmicosin was unchanged for 14 passages.All tilmicosin-resistant mutants of MG and App were cross-resistant to erythromycin,azithromycin,tylosin,kitasamycin and lincomycin.These results indicated that development of resistance to tilmicosin in MG and PM might be a relatively frequent event,so it is necessary to use tilmicosin in treatments cautiously.更多还原