【摘要】 目的构建乙型肝炎病毒免疫吸附柱。方法以琼脂糖凝胶为载体,经活化后键合人抗-HBs-IgG。构建流程为:环氧介质的合成、氨基介质的合成、醛基介质的合成、免疫吸附介质的合成、未反应醛基的封尾、装柱。体外观察其对含HBV和HBsAg血浆的吸附作用。结果活化琼脂糖凝胶与抗-HBs-IgG的键合率为85.07%,患者血浆体外吸附实验结果表明,该免疫吸附柱可吸附、除去离体血浆中58.97%的HBsAg和53.1%的HBV颗粒。结论乙型肝炎病毒免疫吸附柱可吸附除去离体血浆中部分HBsAg和HBV颗粒。更多还原

【Abstract】 Objective To configurate an immunoadsorption column for hepatitis B virus.MethodsAfter activated by epichlorohydrin,the human anti-HBs-IgG is bonded to the carrier of agarose gel.The configuration process is as such:the synthesis of epoxide matrix,the synthesis and activation of amino matrix,the synthesis of aldehydic matrix,the synthesis of immunoadsorption matrix,the endcaping and reduction of unbonded aldehydic,the blocking of unbonded mass and filling the column.Results The bonded rate of activated agarose gel and anti-HBs-IgG is 85.07%.It is showed by the extracorporeal absorbent experiment of patients’ plasma that the immunoabsorption column can absorb and eliminate 58.97% of HBsAg and 53.1% of hepatitis B virus particles in extracorporeal plasma.Conclusions The immunoabsorption column for hepatitis B virus can absorb and eliminate of HBsAg and hepatitis B virus particles in extracorporeal plasma.更多还原