【摘要】 目的:建立测定强力天麻杜仲胶囊中天麻素含量的高效液相色谱方法。方法:采用Agilent Extend C18(250 mm×4.6 mm,5μm)柱,以甲醇-0.1%乙酸溶液(2∶98)为流动相,流速:1.0 mL·min-1;在220 nm波长处检测。结果:天麻素在(0.10~1.20)μg范围内呈线性关系,r=0.999 9;平均加样回收率为97.26%,RSD为2.10%(n=5)。结论:该方法简便快速,准确性和重复性好,可用于强力天麻杜仲胶囊中的天麻素的含量测定。更多还原

【Abstract】 Objective:To develop an HPLC method for determination of gastrodin in Qianglitianmaduzhong capsules.Methods: Analytical column was Agilent Extend C₁₈（250 mm×4.6 mm,5 μm）.The mobile phase consisted of methanol-0.1% acetic acid solution（2∶98）.The flow rate was 1.0 mL·min（-1）.The detector wavelength was set at 220 nm.Result: The calibration curve was linear over the range of（0.10～1.20）μg for gastrodin（r=（0.999 9））.The average recovery（n=5）was 97.26%,RSD was 2.10%.Conclusion: The developed method is convenient rapid and accurate with good reproducibility.It is suitable for the analysis of gastrodin in Qianglitianmaduzhong capsules.更多还原