【摘要】 目的探讨外周血单个核细胞核因子-κB(NF-κB)活性及相关炎性因子白介素-1β(IL-1β)、IL-6、IL-8、肿瘤坏死因子-α(TNF-α)、IL-4和IL-10在小儿多器官功能障碍综合征(MODS)中的作用及临床意义。方法2004-10—2005-06,于广州市儿童医院采用酶联免疫吸附法(ELISA)检测28例MODS患儿第1、3、7天3个时点外周血单个核细胞NF-κB活性,同时采用Luminex TM-100液相分析平台测定血清IL-1β、IL-6、IL-8、TNF-α、IL-4和IL-10质量浓度,并设立对照组20例。结果MODS患儿各时点单个核细胞NF-κB活性、血清IL-6、IL-10明显高于对照组(P<0.05),在存活组呈降低趋势而死亡组维持在高水平(P<0.05),TNF-α显著下降;NF-κB活性与TNF-α负相关(r=0.333,P=0.002),与IL-6正相关(r=0.292,P=0.007)。结论MODS患儿外周血单个核细胞NF-κB活性、血清IL-6、IL-10水平的持续升高预示预后不良。更多还原

【Abstract】 Objective To observe the change of nuclear factor Kappa B(NF-κB) and correlated cytokines(IL-1β、IL-6、IL-8、TNF-α、IL-4 and IL-10) in children with multiple organ dysfunction syndrome(MODS),and to evaluate their potent role in the process of MODS.Methods The activation of NF-κB was measured by enzyme-linked immunosorbent assay(ELISA) in 28 children with MODS at day 1,3 and 7,who were divided into a group of the recovered(n=16) and a group of the dead(n=12).A healthy control group(n=20) was also estaldished.The correlated cytokines were examined by means of LuminexTM-100.Results The activation of NF-κB and levels of IL-6 and IL-10 in children with MODS were higher than those in control group(P<0.05),and they were consistently higher in the dead group than in the recovered group(P<0.05),while the level of TNF-α decreased more significantly in MODS group than in control group.The increased activity of NF-κB was positively correlated with the level of IL-6(r=0.292,P=0.007),but negatively correlated with the level of TNF-α(r=0.333,P=0.002).Conclusion Persistent elevation of NF-κB activation and IL-6 、IL-10 levels correlates with poor outcome in children with MODS.更多还原