【摘要】 目的:建立结肠癌双向电泳技术。方法:利用不同体积的裂斛液提取结肠癌中的蛋白质,并通过不同的蛋白质上样量(1mg,2mg,3mg),不同样品制备方式,进行双向电泳,考马斯亮蓝染色,图谱分析。结果:在等电点3～10,分子量6.5～200ku范围内分离得到蛋白质斑点为,1mg蛋白质上样量为658个蛋白质斑点,2mg为820个,3mg为845个斑点。2mg蛋白质上样量的双向电泳图谱更清晰,分离更好。制备方式2可以满足双相电泳的需要。结论:成功建立了结肠癌蛋白质的双向电泳技术。更多还原

【Abstract】 Objective:To establish a two-dimensional electrophoresis technology tissue protein of colon carcinnma. Methods: Lysis buffer of different volume was taken to extract tissue proteins of colon carcinoma,and different protein quantities (1mg,2mg,3mg) different preparation ways were taken to establish a two-dimensional electrophoresis. Coomassie brilliant blue was applied to stain protein,and patterns were analyzed. Results:With a molecular mass between 6.5～200ku and isoelectric points (pI) from 3～10,1mg proteins obtained 569 protein spots,2mg 786,and 3mg 835. Pattern of 2mg protein and the sample two were the best. Conclusion:A two-dimensional electrophoresis technology on tissue protein of colon carcinoma has been established successfully.更多还原