【摘要】 目的:研究转化生长因子(TGF-β)和信号调节激酶(ERK)在去卵巢大鼠骨组织中的表达及意义。方法:30只2月龄SD雌性大鼠分别造模为假手术组(SHAM组)、卵巢切除组(OVX组)及雌激素替代组(OVX-N组)各10只,用免疫组化法测定成骨细胞中TGF-β和磷酸化ERK的表达并进行定量分析。结果:TGF-β蛋白阳性表达主要在成骨细胞的胞膜上和胞浆内,ERK主要在成骨细胞核内,OVX-N组TGF-β和ERK的阳性表达高于OVX组(P<0.01,0.05),与SHAM组比较差异不明显。结论:TGF-β和ERK可能在绝经后骨质疏松症中起重要调节作用。更多还原

【Abstract】 Objective:To study the expression and significance of TGF-β and ERK in the bone tissues of ovariectomized rats.Methods:Osteoporosis models of rats were established by bilateral ovariectomy.Thirty female Wistar rats were equally randomized into ovariectomized group(OVX),sham operation group and ovariectomized and nilestriol-treated group(OVX-N).The expression of ERK and TGF-β was detected by immunohistochemical method and was quantitatively analysed.Results:TGF-β was mainly expressed on the osteoblast cytomembrane and incytoplasm,and phosphorylated ERK was mainly expressed in osteoblast nucleus.The positive expression of TGF-β and phosphorylated ERK in OVX-N group was significantly increased as compared with that in OVX group(P<0.01),but there was no significant difference between OVX-N group and sham group.The expression of TGF-β and ERK in OVX-N group was higher than in OVX group(P<0.05).There was no significant difference between OVX-N group and sham group.Conclusion:The expression of TGF-β and ERK may play some important roles in the pathogenesis of primary osteoporosis.更多还原