【摘要】 目的研究不同浓度188Re-DTPA-DG(188Re标记二乙三胺五乙酸-葡糖胺)对人肺癌细胞A549增殖的影响,探讨其作用机制。方法将人肺癌细胞A549分成3组,实验组(188Re-DTPA-DG组)、对照组(含188ReO4-淋洗液、缓冲液及等量的DTPA-DG)及生理盐水组(含生理盐水及等量缓冲液),前两组均设三种不同浓度,148、296、444MBq/L。观察细胞形态,采用四氮唑盐比色法(MTT法)评价188Re-DTPA-DG对癌细胞A549增殖的影响。结果188Re-DTPA-DG导致的细胞损伤比188ReO4-明显;随着药物浓度增加,188Re-DTPA-DG导致的损伤越显著。结论188Re-DTPA-DG对肿瘤细胞增殖有较明显的抑制效应,其机制可能是由于188Re-DTPA-DG进入到细胞内,其辐射效应更能导致肺癌细胞损伤。更多还原

【Abstract】 Objective To investigate the influence of 188Re-DTPA-DG on human pulmonary carcinoma cell line and elucidate its possible mechanism.Methods A549 cells were cultured,they were divided into 3 groups,namely,188Re-DTPA-DG,control,saline group,and the former two groups were divided into 3 groups respectively by 3 different concentrations (148 、296、444MBq/L),then they were acted on respectively through a night.Cell growth was assessed with MTT before the appearance of the cells was watched with microscope.Results The ability of growth was inhibited in about 16 hours,and the inhibiting ratio was higher in 188Re-DTPA-DG group than in control group (P<0.001).In 188Re-DTPA-DG group,when there is a higher concentration,the inhibiting ratio also becomes higher;while in 188 ReO-4 group,it does not.Conclusion The influence of 188Re-DTPA-DG is superior to 188ReO-4 on human pulmonary carcinoma cells,which may contribute to the mechanism that 188Re-DTPA-DG can be involved in tumor cells while 188ReO-4 can not,and the cell injury extent occurred by them is different.更多还原