【摘要】 根据Ⅰ型鸭病毒性肝炎ZJ/06强毒株的测序结果,在其基因组3D基因区设计合成一对可扩增238 bp的特异性引物,成功的建立了检测DHV-Ⅰ的RT-PCR方法,并确定了其特异性与敏感性,对DHV-Ⅰ分离毒株和临床病料进行RT-PCR检测均得到阳性扩增结果,而作为阴性对照的常见4种鸭病病原:鸭瘟病毒、鸭产蛋综合症病毒、鸭副粘病毒、鸭里默氏杆菌均未扩增到任何片断。利用BHK-21细胞、SPF鸡胚对DHV-Ⅰ临床病料进行病毒分离鉴定,结果表明建立的DHV-Ⅰ的RT-PCR检测方法具有快速、特异、敏感的特点。更多还原

【Abstract】 According to the results of DNA sequencing of duck hepatitis virus(DHV-Ⅰ) ZJ/06 virulent strain,a pair of specific primers which could amplify 238 bp fragment in gene region of genome 3D were designed and developed,a RT-PCR method detecting DHV-Ⅰ was developed successfully,and the specific and susceptibility of DHV-Ⅰ were determined.A specific 238 bp fragment could be amplified from virulent strains and clinic samples of DHV-Ⅰ by RT-PCR method,but no bands were amplified from four kinds of normal duck diseases: duck plague virus(DPV),duck egg drop syndrome virus(DEDSV),duck paramyxovirus(DPV) and Riemerella anatipestifer from duck(DRA).Some clinical samples of DHV-Ⅰ were isolated and identified using BHK-21 cell and SPF chicken embryo,respectively.The results showed that the method of RT-PCR setup for detecting DHV-Ⅰ had the character of fast,specific and sensitive.更多还原