【摘要】 目的研究中药三脉血注射液对急性心肌梗死大鼠VEGF、bFGF的影响及其促血管新生作用。方法取健康Wistar大鼠50只,雌雄各半,体质量200～250 g,随机分为四组:假手术组(A组),生理盐水对照组(B组),三脉血大剂量组(C组),三脉血小剂量组(D组),14 d后处死动物,进行免疫组化检测梗死-非梗死交界处区VEGF、bFGF表达,并于显微镜下观察其毛细血管数。结果与B组相比C、D组VEGF、bFGF表达增加(P<0.05),毛细血管数增加(P<0.05);C组和D组相比,VEGF、bFGF表达及毛细血管数量差异无统计学意义(P>0.05)。结论三脉血注射液可以促进急性心肌梗死大鼠缺血心肌分泌VEGF、bFGF,并促进毛细血管生成,发挥心肌保护作用。更多还原

【Abstract】 Objective To study the effects of Sanmaixue injection on the angiogenisis and the ex- pression of vascular endothelial growth factor(VEGF)and basic fibroblast growth factor(bFGF)on ische- mic myocardium of rats.Methods 50 Wistar rats weighting 200～250 g were randomly devided into 4 groups:sham operation group(group A),NS group(group B),Sanmaixue high dose group(group C },San- maixue low dose group(group D).After 14 days,all rats were killed.The expression of VEGF,bFGF on ischemic myocardium were detected with immunohistochemical staining,and microvascular count were cal- culated by microscope with HE.Results Compared with group B,the expression of VEGF on group C and D were increased(P<0.05),microvascular count on group C and D were increased(P<0.05);the difference of VEGF,bFGF and MVC between group C and D were not found(P>0.05).Conclution Sanmaixue injection may promote the expression of VEGF,bFGF and angiogenisis on ischemic myocardi- um of rats,and play a part of protection on ischemic myocardium.更多还原