【摘要】 目的探讨细胞外间质韧黏素-C(TN-C)的分解与非小细胞肺癌(NSCLC)术后复发的关系,以及TN-C分解的分子机制。方法收集63例Ⅰ期NSCLC患者的组织标本,以Western blot测定TN-C蛋白的表达,明胶酶谱法测定基质金属蛋白酶(MMPs)的活性。结果63例患者中,12例检测到TN-C的分解片段。有TN-C分解组肺癌组织与正常肺组织MMP-2活性之比为3.5±0.4,明显高于无TN-C分解组(1.5±0.4,P<0.001)。有无TN-C分解与肺癌的复发、转移有关,可检测到小分子TN-C片段者复发、转移率高(P<0.001)。无TN-C分解患者的5年生存率为73.9%,高于有TN-C分解的患者(45.8%,P=0.028)。无TN-C分解患者的4年和10年无复发生存率分别为82.1%和76.6%,高于有TN-C分解的患者(P<0.001)。结论TN-C分解片段是NSCLC术后复发、转移的有效指标,MMP-2可能是肺癌组织中分解TN-C的重要蛋白酶。更多还原

【Abstract】 Objective To study the correlation of tenascin-c(TN-C) degradation with relapse and/ or metastasis in stage- I non-small cell lung cancer ( NSCLC) in order to search for a potential biomarker for predicting recurrence, and also to investigate the molecular mechanism of TN-C degradation. Methods The fragment of TN-C in 63 surgically treated stage- I NSCLC was detected by Western blotting, and the activity of matrix metalloproteinases( MMPs) was also examined by gelatin zymography. Results TN-C degradation fragment was positively detected in 12 of 63 patients, and 9 of these 12 patients (75. 0% ) were found to develope recurrence during follow-up. The recurrence-free survival at 4 years was 28. 1% in patients with positive TN-C degradation versus 82. 1% in those without( P <0. 001) , and which was 76. 6% at 10 years in the patients without TN-C degradation. The activity of MMP-2 in the patients with positive TN-C degradation was also found to be significantly higher than that in the patients without (P<0.001). Conclusion Tenascin-c degradation fragment may be a reliable biomarker for predicting recurrence and/or metastasis in the early NSCLC, and matrix metalloproteinase-2 may be a responsible proteinase for degradation of tenascin-c.更多还原