【摘要】 目的探讨慢性肝病患者乙型肝炎病毒C基因启动子变异(HBV BCP)与血清白细胞介素10(IL-10)、12(IL-12)、肿瘤坏死因子α(TNF-α)、γ-干扰素(IFN-γ)及病毒含量(HBV DNA)的关系。方法采用PCR微板核酸杂交结合ELISA检测显示技术,对176例HBV慢性感染者(慢性乙型病毒性肝炎轻、中、重度,肝炎肝硬化,慢性重型肝炎和原发性肝癌)血清进行检测HBV BCP区核苷酸(nt)1762碱基A→T和1764碱基G→A联合突变;采用双抗体夹心ELISA检测技术,检测患者血清细胞因子(IL-10、IL-12、TNF-α和IFN-γ)水平。结果HBVBCP变异阳性组、阴性组间对细胞因子(IL-10、IL-12、TNF-α和IFN-γ)及HBV DNA复制水平的影响差异有统计学意义(P<0.05),BCP变异阳性组的血清IL-10(80.96±30.86vs72.11±24.19 mg/L)I、L-12(41.33±15.10vs35.98±14.47 mg/L)、TNF-α(56.04±27.05vs38.01±10.49 mg/L)、IFN-γ(19.81±12.29vs16.55±8.99mg/L)和HBV DNA(108.2478±0.9826vs105.8876±1.4822拷贝/ml)的水平明显高于BCP变异阴性组。结论HBV BCP变异可导致血清IL-10I、L-12、TNF-α、IFN-γ和病毒复制水平增高。更多还原

【Abstract】 OBJECTIVE To study the correlation of hepatitis B virus(HBV)basic core promoter(BCP) mutation with the interleukins(IL-10,IL-12),tumor necrosis factor(TNF)-α,interferon(IFN)-γ,as well as HBV DNA contents in patients with chronic hepatitis B virus infection.METHODS(1) Project subject: 176 patients(chronic hepatitis B with mild,moderate and severe degree;liver cirrhosis,chronic fulminant and hepatocellular carcinoma(HCC)) with chronic hepatitis B virus infection were studied.(2) Project methods: ① The A to T mutation at nucleotide 1762 and G to A mutation at nucleotide 1764 were determined by the method of polymerase chain reaction(PCR) microplate hybridization ELISA in these patients.② The serum cytokines(IL-10,IL-12,TNF-α and IFN-γ) of these patients were measured by specific-ELISA.RESULTS The serum levels of cytokines(IL-10(80.96±30.86 vs 72.11±24.19 mg/L),IL-12(41.33±15.10 vs 35.98±14.47 mg/L),TNF-α(56.04±27.05 vs 38.01±10.49 mg/L),IFN-γ(19.81±12.29 vs 16.55±8.99 mg/L))and HBV DNA contents(108.2478±0.9826 vs 105.8876±1.4822copies/ml) in BCP mutant group were significantly higher than that in non-mutant group(P<0.05).CONCLUSIONS The mutants in HBV BCP might enhance the serum levels of cytokines(IL-10,IL-12,TNF-α and IFN-γ),which could lead to the replication of HBV DNA.更多还原