【摘要】 目的研究细胞间黏附分子1(ICAM-1)与血管细胞黏附分子1(VCAM-1)在心脏移植慢性排斥反应中的表达及作用。方法采用供体脾细胞(SPC)和环磷酰胺(CP)预处理移植受体,然后行异位心脏移植术,免疫组织化学检测移植心脏中 VCAM-1和 ICAM-1的表达。结果 SPC 和 CP预处理后,移植心脏中 ICAM-1、VCAM-1的表达水平明显减低,而 ICAM-1和 VCAM-1在急性排斥反应和环孢素 A(CsA)治疗组中的表达明显增强(均 P<0.05)。急性排斥反应与 CsA 治疗组比较差异无统计学意义。结论 ICAM-1和 VCAM-1的表达水平与心脏移植排斥反应的程度密切相关。更多还原

【Abstract】 Objective To investigate the expression of intercellular adhesion molecule-1 (ICAM- 1) and vascular cell adhesion molecule-1 (VCAM-1) in allograft with chronic rejection (CR) after heart transplantation.Methods Seventy-two SD rats receiving transplantation of the heats of 71 Wistar rats were divided into 4 equal groups:Group A,undergoing heterotopic cervical heart transplantation (transplanted with donor’s heart subcutaneously at the neck) and receiving none intervention,and with the transplanted hearts taken out 3 d after transplantation to the end of CR;Group B,injected intravenously with the splenocytes (SPCs) of the donors on day 0,injected with cyclophomide (CP) on d2,transplanted with the donor hearts on d15,with the transplanted hearts taken out 15-120 days after transplantation;Group C, transplanted with the donor’s heart and injected intraperitoneally with cyclosporine A (Cs A) 10 mg/kg every other day for 8-10 times,and with the transplanted hearts taken out 60 d after transplantation to the end of CR;and Group D,injected intravenously with the SPCs of the donors on day 0,injected with CP on d2,transplanted with the donor hearts on d15,with the transplanted hearts taken out 150-420 days after transplantation.Immunohistochemical method was used to detect the expression of ICAM-1 and VCAM-1 in the allografts.Results The cardiac allograft survival time of Group B was the longest.Less ICAM-1 and VCAM-1 expression and lymphocyte infiltration were observed in SPC and CP-pretreated group.Reversely, protein expression levels of ICAM-1 and VCAM-1 were both high and significant lymphocyte infiltration was seen in Groups B and D.There was no significant difference in the expression of ICAM-1 and VCAM-1 between Groups A and C (P>0.05).Conclusion The expression levels of ICAM-1 and VCAM-1 are associated with the occurrence and development of rejection and have positive correlation with the severity of graft rejection.Determination of ICAM-1 and VCAM-1 can predict the function of allograft and provide evidence for early diagnosis and prevention of CR.更多还原