【摘要】 目的探讨大鼠脊髓进行性压迫损伤细胞凋亡、半胱氨酸蛋白水解酶(caspase-12)表达变化规律及其关系。方法随机选用健康成年 Wistar 大鼠120只,分为两组:手术组和对照组各60只,每个时间点各10只,按自行设计的方法作脊髓压迫模型;对照组,仅做脊髓显露。分别于术后1、3、7、14、21、28 d 观察脊髓受压后细胞凋亡及 caspase-12的表达变化系。结果在各压迫组神经细胞出现凋亡,凋亡的发生率在1、3、7、14、21、28 d 分别为12.5%±2.3%、13.0%±3.6%、17.2%±4.3%、29.4%±4.4%、36.1%±6.5%、2.3%±7.9%,并伴随 caspase-12的表达增强而增加,14、21、28 d 组间比较差异有统计学意义(均 P<0.05)。结论 caspase-12可能参与了脊髓进行性压迫缺血所致的细胞凋亡。更多还原

【Abstract】 Objective To investigate thee apoptosis and caspase-12 expression in progressive compression of spinal cord(PCSC).Methods 120 adult Wistar rats were randomly divided into 2 equal groups,experimental group,undergoing operation so as to establish PCSC models,and control group, undergoing sham operation.1,3,7,14,21,and 28 days after the operation,5 rats from each group were anesthetized with their spinal cords taken out.TUNNEL method was used to observe the apoptosis of the neurons in the compressed segments.Another 5 rats from each group at different time points were anesthetized with their spinal cords as well.Immunohistochemistry was used to detect the mRNA expression of caspace-12 in the compressed segments.Western blotting was used to detect the protein expression of caspace-12.Results The apoptotie rates of neurons and gliocytes 1,3,7,14,21,and 28 days after were 12.5%±2.3%,13.0%±3.6%,17.2%±4.3%,29.4%±4.4%,36.1%±6.5%,and 2.3%± 7.9% respectively,with significant differences among the values 14,21,and 28 days and those at other time points after the operation(all P<0.05).Immunohistochemistry showed that the number of caspace-12 positive neurons increased since 1 day after,and became remarkably high 14,21,and 28 days after with significant differences among different time points(all P<0.05).Western blotting showed that the protein expression of caspace-12 was low 1,3,and 7 days after,and peaked 14 days after,and then gradually decreased,however,the expression levels 21 and 28 days after were still significantly higher then those 1, 3,and 7 days after(all P<0.05).Conclusion Caspase-12 is involved in the apoptosis of neurons in PCSC.更多还原