【摘要】 目的探讨摄食行为及能量代谢相关的重要蛋白质,黑皮素4受体(MC4R)基因变异与中国人肥胖的相关性。方法选取563例上海地区中国人,其中 BMI≥30 kg/m~2者258例,BMI<23 kg/m~2者305例,用 DNA 测序法检测 MC4R 基因 nt-216C/T、nt-178A/C 和 Val103Ile 变异。并测定所选人群身高、体重、腰围、臀围、股围、舒张压和收缩压。对所选人群进行口服糖耐量试验,测定空腹和糖负荷后120 min 血糖水平,并测定空腹血脂谱。以生物电阻抗法测定体脂含量。结果 (1)中国人中 nt-216C/T、nt-178A/C 和 Val103Ile 变异频率均小于5%;(2)Logistic 回归分析见到,MC4R 基因Val103Ile 变异是肥胖的独立相关因素(OR=0.414,P=0.040),Ile 等位基因频率在肥胖患者中较低。(3)在正常体重组和肥胖组中分别比较 Val/Val 纯合子与 Ile 携带者体脂参数、血糖、血脂、血压,差异无统计学意义。结论 MC4R 基因 Val103Ile 变异与中国人肥胖相关。更多还原

【Abstract】 Objective To investigate the association between the polymorphisms of melanocortin-4 receptor (MC4R) gene and obesity.Methods The genotypes of three polymorphisms,nt-216C/T,nt- 178A/C,and Val103Ile,were determined through DNA sequencing in 563 Chinese from Shanghai, including 258 individuals with body mass index (BMI) over 30 kg/m~2 and 305 individuals with BMI less than 23 kg/m~2.Height and body weight were measured to calculate BMI.Blood pressure was measured. Waistline,hipline,and femoral waist to calculate the waist-to-hip ratio and waist-to-femoral ratio were measured.Oral glucose tolerance test was conducted to detect the plasma glucose level during fasting condition and 120 minute after glucose loading.The levels of serum lipids,including total cholesterol, triglyceride,high-density lipoprotein cholesterol(HDL-C),and low-density lipoprotein cholesterol.Results (1) The frequencies of nt-216C/T,nt-178A/C,and Vall03Ile were all less than 5%.(2) Logistic regression showed that the Val103Ile variant was an independent risk factor for obesity(OR=0.414,P= 0.040).The frequency of Ile was less in the obese individuals compared with the controls.(3) In the controls and obese individuals,no association was detected between the genotypes and phenotypes. Conclusion Val103Ile variant of MC4R gene is associated with obesity in Chinese.更多还原