【摘要】 目的通过建立3T3中性红成纤维细胞光毒替代实验,研究体外替代方法替代动物实验及在化妆品安全性评价中取代动物光毒性实验的可行性。方法选用24种已知光毒性化学物质(15种阳性、9种阴性)和20种待检特殊用途化妆品,测量 Bal/c3T3细胞经化学物质和紫外线照射联合作用后的平均光效应强度和光刺激因子,与整体动物皮肤光毒性结果比较。结果 3T3体外替代实验对24种已知光毒性化学物质检测结果为14种阳性和10种阴性,20种化妆品为阴性,经校正 x~2检验与既往光毒性结果比较,差异无统计学意义。结论本次研究成功建立了3T3中性红成纤维细胞光毒替代实验方法,该方法可取代豚鼠皮肤光毒性实验对化妆品终产品进行安全性评价。更多还原

【Abstract】 Objective To establish the 3T3 mouse fibroblast neutral red uptake(NRU-PT) phototoxicity test method,and evaluate the practicality of the method in detecting potential phototoxicity of the cosmetic products.Methods Fifteen phototoxic and 9 non-phototoxic chemicals were tested in our laboratories,the phototoxic potential of the test chemicals was evaluated in a prediction model in which either the photoirritation factor(PIF)or the mean photo effect(MPE)was compared with the coherence and sensitivity of the method.20 kinds of functional cosmetics were detected and the results were analyzed by the 3T3 NRU-PT in vitro and Guinea pig skin phototoxicity test(in vivo)Results Both PIF and MPE of the chemicals were highly reproduced,and the correlation between in vitro and in vivo data was almost perfect. All the non-phototoxic provided a negative result,while 14 of the 15 phototoxic tested chemicals gave clear positive results.For cosmetics,the correlation between in vitro and in vivo data was consistent.Conclusion The 3T3 NRU PT test was established successfully,it should be used as a good alternative method for assessing the phototoxic potential of the chemicals and cosmetics in China.更多还原