【摘要】 目的了解调查年份不同种类海、水产品中分离的霍乱弧菌的血清学分型、噬菌体-生物分型及毒力因子携带情况,分析海水产品污染状况与疫情的关系,为疫情预测和制定防治对策提供参考。方法应用血清学分型及噬菌体-生物分型方法分析所分离菌株的生物表型,应用 PCR 方法检测毒力基因的携带情况。结果检测的64株菌株中0139型菌株占48.44%(31/64),01群小川型占31.25%(20/64),01群稻叶型占20.31%(13/64);O1群噬菌体-生物分型显示均为非流行株;对61株菌毒力基因检测结果显示:O139霍乱弧菌毒力基因的检出率(83.33%)远高于 O1群小川型霍乱弧菌(15.38%)和 O1群稻叶型霍乱弧菌(11.1l%)。结论 O139型霍乱弧菌主要存在于甲鱼、甲鱼养殖水及甲壳类海水产品中,而且多为产毒株,这些海、水产品是预防和控制霍乱疫情的重要环节。更多还原

【Abstract】 Objective To investigate the serologic type,phage-biotype and toxic factor of Vibrio cholerae isolated from different sea products,analyze the relation between the Vibrio cholerae in sea products and cholera epidemiology,and provide references for forecasting cholera epidemic situation and drawing out a preventing plan.Method The biotype of strains isolated was analyzed by using type and phage-biotype serological methods.The toxic gene was detected by PCR.Results The constituent ratio of V.cholerae O139,Ogawa and Inaba were,respectively,48.44%,20.31% and 31.25% in 64 strains of V.cholerae. The result of phage-biotype showed that the 26 strains of V.cholerae O1 were all non-epidemic strains.The result of toxic gene detecting showed that positive rate of V.cholerae O139 was higher than those of Ogawa and Inaba.Conclusion The positive rate of toxic gene in V.cholerae 0139 was high and the V.cholerae O139 was mainly in turtle,breed aquatics water and crustacean,so these sea products were the important sectors in cholera prevention and control.更多还原