【摘要】 目的了解北京市外来人口中HIV-1亚型的特点和流行规律。方法随机采集北京市2006年外来人口中新确证HIV-1感染者的抗凝全血标本80份,分离血浆,提取病毒RNA,用套式聚合酶链反应扩增病毒gag基因,并进行序列测定和亚型分析。结果系统进化分析确定北京市外来人口HIV-1毒株属于8个亚型,分别为B亚型4份,泰国B亚型15份,C亚型1份,CRF01_AE亚型5份,CRF02_AC亚型1份,CRF07_BC亚型29份,CRF08_BC亚型3份,CRF15₀1B亚型1份。结论北京市外来人口中已存在8种HIV-1亚型和流行重组型,应该加强对HIV-1亚型变异的监测。更多还原

【Abstract】 Objective To investigate the subtype distribution and sequence characteristics of HIV-1 strains prevalent in Beijing during 2006, and to analyze the relationship between distribution of HTV-1 subtypes and transmission routines. Methods We collected the blood samples from 80 HIV-1 infected individuals from immigration in Beijing during 2006 and separated plasma specimens. RNA was extracted and the gag gene was amplified by RT-PCR and nest-PCR. The PCR products were sequenced directly and phylogenetic analysis of gag gene was performed using the MEGA2 software. Results Among 80 HIV-1 plasma samples,59 gag HIV-1 gene fragments were amplified and analyzed. Eight HIV-1 subtypes or circulating recombinant forms（CRF） of HIV-1 including B（4 strains）, Thai B（15 strains）, C（1 strains）, CRP01 AE （5 strains）, CRF02 AG（1 strains）, CRP07_BC （29 strains）, CRF08_BC（3 strain）,CRF15 01B （1 strains） were identified circulating in Beijing. The gene divergences inside the subtypes were different, with 6.8 % , 25.4 % , 1.7 % , 5.5 % , 1.7 % , 49.2 % , 5.1 % and 1.7% in subtype B, Thai B, C, CRF01 AE, CRF02 AG, CRF07 BC, CRF08 BC, CRF15₀1B respectively. Subtypes CRF07 BC were predominant in Beijing immigration account for 49.2% among 59 samples. Conclusion Eight HIV-1 subtypes exist in Beijing and the surveillance of HIV-1 gene variation should be paid more attention.更多还原