【摘要】 目的调查猪HEV的基因型和亚型。方法收集新疆南部和广西柳州地区3月龄以下猪粪便标本134份,用巢式逆转录聚合酶链反应法(RT-nPCR)检测戊型肝炎病毒核糖核酸(HEV RNA),对RT-nPCR阳性扩增产物进行克隆测序,用Vector NTI Suite 9.0和TreeView软件与目前新的分型方法所选参考序列进行核苷酸序列相似性比较和生物进化树分析。结果新疆和广西猪粪便标本的HEV RNA阳性率分别为3.64%(2/55)和3.80%(3/79),命名为XJ3-2、XJ3-6、GX3-1、GX5-2和GX6-3。5株猪HEV的开放阅读框架1(ORF1)的部分核苷酸序列与Ⅰ、Ⅱ、Ⅲ和Ⅳ型HEV的相似性分别为74%～77%、76%～77%、73%～81%和82%～98%。同时对开放阅读框架2(ORF2)部分核苷酸序列进行了分析,GX3-1和GX6-3与Ⅳb亚型相似性最高,为88%～95%。GX5-2、XJ3-2和XJ3-6与Ⅳa亚型相似性最高,为90%～96%。结论新疆和广西5株猪HEV均属于基因型Ⅳ,新疆2株为Ⅳa亚型,广西1株为Ⅳa,另2株为Ⅳb亚型。未检测到HEV基因Ⅰ型和Ⅲ型。更多还原

【Abstract】 Objective To investigate the genotype and subgenotype of swine hepatitis E virus(HEV). Methods One hundred and thirty-four fecal specimens of swine under 3 months old were collected from the South Xinjiang and Liuzhou,Guangxi.HEV RNA was amplified by a reverse transcription nested polymerase chain reac- tion(RT-nPCR),and RT-nPCR products were cloned and sequenced.The nucleotide sequence comparison and phylogenetic analysis of HEV isolated from swine were carried out by using the Vector NTI Suite 9.0 and TreeView softwares.Results HEV RNA positive rates were 3.64%(2/55)in Xinjiang swine,and3.80%(3/79)in Guan- gxi swine.A total of 5 HEV RNA strains were named as XJ3-2,XJ3-6,GX3-1,GXS-2 and GX6-3.The homoge- nous profile of HEV ORF1 nucleetide sequences between XJ3-2,XJ3-6,GX3-1,GX5-2,GX6-3 and genotypeⅠ-Ⅳwas 74%-77%,76%-77%,73%-81% and 82%-98%,respectively.The nucleotide homology was 90%- 96% between ORF2 of GX5-2,XJ3-2,XJ3-6 and HEV genotypeⅣa,and 88%-95% between ORF2 of GX3-1, GX6-3 and genotypeⅣb.Conclusion The 5 HEV strains from both Xinjiang and Guangxi were classified as HEV genotypeⅣ.However,2 HEV strains isolated from Xinjiang swine belonged to subgenotypeⅣa,and HEV from Guangxi was identified asⅣa(1 strain) andⅣb(2 strains),respectively.HEV genotypeⅠand genotype m were not detected in swine feces obtained from Xinjiang and Guangxi.更多还原