【摘要】 目的探讨胃癌组织中缺氧诱导因子-1α(HIF-1α)及胰岛素样生长因子-Ⅱ(IGF-Ⅱ)mRNA 的表达水平及其与血管生成和预后的关系。方法采用原位杂交检测118例胃癌组织中 HIF-1α mRNA 和 IGF-Ⅱ mRNA 的表达情况,用免疫组化 SP 法标记 CD34单克隆抗体,计算肿瘤微血管密度(MVD)。结果 HIF-1α mRNA 和 IGF-Ⅱ mRNA 在胃癌组织中的阳性表达率分别为49.2%和47.4%。且与胃癌临床侵袭转移病理指标均相关。HIF-1α mRNA 和 IGF-Ⅱ mRNA 阳性表达者的MVD 值,均高于阴性表达者;HIF-1α mRNA 和 IGF-Ⅱ mRNA 表达呈正相关,MVD 分别与 HIF-1αmRNA 和 IGF-Ⅱ mRNA 的表达水平呈正相关。HIF-1α mRNA 和 IGF-Ⅱ mRNA 表达阳性及 MVD 值≥41.5个/0.72 mm~2的患者的平均生存时间及5年生存率均低于表达阴性及 MVD 值<41.5个/0.72mm~2者。结论 HIF-1α与 IGF-Ⅱ对胃癌的浸润转移,特别是对肿瘤血管形成具有重要作用,可作为预测患者预后和指导治疗的新途径。更多还原

【Abstract】 Objective To investigate relationship between expressions of hypoxia inducible factor- 1α(HIF-1α)and insulin-like growth factor-Ⅱ(IGF-Ⅱ)as well as their correlation with angiogenesis and prognosis in gastric carcinoma.Methods HIF-1α and IGF-Ⅱ mRNA expression were analyzed using in situ hybridization,microvessel density(MVD)was determined by anti-CD34 immunostaining in 118 cases gastric carcinoma.Results The positive rates of HIF-1α mRNA and IGF-Ⅱ mRNA were 49.2% and 47.4%, respectively.In stage T3-T4 cases,positive rates of HIF-1α and IGF-Ⅱ mRNA expression,the frequencies of vessel invasion,lymph node and distant metastasis were significantly higher than those in stage T1-T2 cases.The mean MVD in stage T3-T4 tumors,vessel invasion,lymph node metastasis and distant metastasis were significantly more frequent than those in stage T1-T2 tumors.The mean MVD in tumors with positive HIF-1αand IGF-Ⅱ mRNA expression was significantly higher than those in tumors without HIF-1α and IGF-Ⅱ mRNA expression.The expression of HIF-1α was positively correlated with IGF-Ⅱ mRNA. There were positive correlations between MVD and expression of HIF-1α and IGF-Ⅱ mRNA,respectively. The mean survival time and 5-year survival rate in cases with positive HIF-1α and VEGF expression and MVD value≥41.5 were significantly shorter than those in cases with negative HIF-1α and VEGF expression and MVD value<41.5.Conclusions HIF-1α and IGF-Ⅱ play an important role in tumor invasion and metastasis,especially in tumor angiogenesis.So test of the expression of HIF-1α and IGF-Ⅱ may act as a useful index of treatment and prognosis.更多还原