【摘要】 目的通过观察不同剂量二甲磺酸乙烷(EDS)对成年大鼠Leydig细胞的杀伤效应,确定EDS的最佳剂量。方法6个月龄SD成年雄性大鼠42只,随机分为EDS处理组、溶酶对照组和正常对照组,其中EDS处理组按照剂量的不同设置40、60、75、90和130 mg/kg体重5个亚组,每个亚组含6只大鼠。腹腔内注射给药,注射EDS后3、7和14d处死大鼠,取出睾丸组织,行苏木素-伊红(HE)染色光镜下组织学观察和P450scc免疫组织化学观察及灰度分析。结果正常对照和溶酶对照组Leydig细胞形态、数目正常,曲细精管内各级生精细胞排列有序,无紊乱现象。EDS处理3d后,130 mg/kg体重剂量组的2只大鼠死亡,其他剂量组均观察到Leydig细胞的减少,其中40 mg/kg体重剂量组的减少程度不明显;EDS处理后7d,曲细精管内生精细胞出现排列紊乱的现象,以90 mg/kg体重剂量组更为明显;EDS处理后14d,60 mg/kg体重剂量组出现一种核圆形、核染色淡、体积较大的新形成Leydjg细胞,而在75 mg/kg体重剂量组没有发现此细胞。P450scc免疫组织化学与光镜结果一致。灰度分析结果显示,EDS处理后3及7 d,各剂量组与正常对照比较差异均有统计学意义(P<0.05);60 mg/kg体重剂量组14与3、7d比较差异有统计学意义(P<0.05),且与正常对照差异无统计学意义(P>0.05)。结论EDS确实能杀死成年大鼠Leydig细胞,60 mg/kg体重的剂量可以更好的模拟青春期Leydig细胞增殖分化过程。更多还原

【Abstract】 Objective To determine the optimal dose of EDS that could modify the proliferation and differentiation of Leydig ceils at pubertain adult rats by injecting different doses of EDS.Methods A- dult SD rats received a single intraperitoneal injection of EDS at a dose of 40,60,75,90,130 mg/kg re- spectively.The vehicle control received injection of DMSO,and the blank control was injected nothing. Rats were killed on day 3,7 and 14 after initial treatment.The testis were collected for stereology,immuno- histochemistry of P450sec.Results Three days after EDS treatment,Leydig cells were almostly killed,and there were no cells stained for P450scc in testis interstitial tissues.Forteen days after EDS treatment,a new Leydig cell population in the group of 60 mg/kg were found.The changes in P450scc enzyme activities co- incided with the results of stereology.Three days and 7 days after EDS treatment,the number of positive cells for P450scc was reduced in all EDS-treated groups as compared with the blank control(P<0.05); In the group of 60 mg/kg,the number of positive cells for P450sce 14 days after EDS treatment was more than that 3 days and 7 days after EDS treatment(P<0.05),but not significandy different from than in the blank control(P>0.05).Conclusion The EDS can kill the Leydig cells of adult rats actually.The dose of 60 mg/kg can simulate the process of Leydig cells development at pubertal stage optimally.更多还原