【摘要】 目的探讨邻苯二甲酸二丁酯(DBP)母体暴露对胚胎期大鼠睾丸蛋白表达谱的影响。方法孕SD大鼠10只,随机分为2组,妊娠14～18 d,实验组和对照组分别予DBP每日800 mg/kg体重、大豆油5 ml/d灌胃,第19天取出子鼠睾丸,每组各3例样本,提取总蛋白,行二维凝胶电泳分离和质谱分析。结果筛选出23个差异表达蛋白(t≥2.831,P<0.05),经蛋白数据库检索,共鉴定出差异蛋白14个(评分>50,P<0.05),这些蛋白主要参与细胞凋亡、细胞增殖与分化和信号转导等。结论蛋白质组学是研究DBP的生殖系统发育毒性的有效方法。更多还原

【Abstract】 Objective To isolate and identify differential expression proteins in embryonic rat testis after maternal exposure to di(nbutyl) phthalate (DBP).Methods Ten pregnant rats were randomly divided into two groups and were administered with DBP by gastric intubation at a dose of 800 (group A) or 0 mg/(kg·d) (group B) from the 14th day to the 18th day of pregnancy.The embryonic testes were taken out at the 19th day of pregnancy,and total testis proteins were extracted.The proteins were separated by two-dimensional gel electrophoresis and identified by mass spectrometry.Results Twenty-three differ- entially expressed protein spots (t≥2.831,P<0.05) were and 14 of them were identified by mass spec- trometry and tested in the SwissProt database (score>50,P<0.05).These differentially expressed pro- teins were mainly related to apoptosis,cell proliferation and differentiation,signal transduction,etc.Con- clusion Proteomics provides an effective method for studying the developmental toxicity of DBP to male reproductive development.更多还原