【摘要】 目的观察奥美拉唑对大鼠减体积肝移植肝细胞再生的影响。方法建立大鼠减体积肝移植模型,实验组移植后即时给予奥美拉唑,对照组予生理盐水。两组分别于肝移植术后分为5组(n=8),观察术后3、5、7、10、14 d血清丙氨酸转氨酶、天冬氨酸转氨酶值、移植肝重/供肝减体积前全肝重比值、移植肝细胞有丝分裂指数(MI)、增殖细胞核抗原(PCNA)表达指数、溴脱氧尿嘧啶核苷(BrdU)掺入指数及血清胃泌素值。结果大鼠减体积肝移植术后5 d肝细胞再生达高峰,实验组的再生活性显著高于对照组,MI为(2.54±0.24)%和(1.71±0.16)%(P<0.01)、PC- NA指数为(26.96±2.09)%和(18.73±1.94)%(P<0.01)、BrdU指数为(10.24±1.11)%和(5.75±0.88)%(P<0.01)。术后7 d,实验组和对照组移植肝重/全肝重比值分别为(76.3±1.6)%和(71.2±1.0)%(P<0.05),血清胃泌素水平分别为(441.9±25.9)ng/L和(292.9±14.2)ng/L(P<0.05)。术后14d,实验组和对照组移植肝重/全肝重比值分别为(94.5±1.7)%和(86.9±1.5)%(P<0.01),血清胃泌素水平分别为(487.8±29.4)ng/L和(291.7±21.6)ng/L (P<0.01)。实验组和对照组血清ALT、AST值差异无统计学意义。结论奥美拉唑能促进减体积肝移植术后的肝细胞再生,其作用可能与胃泌素分泌增高有关。更多还原

【Abstract】 Objective To investigate the effects of omeprazole on liver regeneration after re- duced-size liver transplantation in rats.Methods After reduced-size liver transplantation 80 rats were di- vided into two groups:experimental group and control group.The rats in experimental group and control group received omeprazole and NS respectively.In each group (n=8),the liver function (serum ALT, AST),the ratio of remnant liver weight vs total liver weight,the mitotic index (MI) of the transplanted liv- ers,the proliferating cell nuclear antigen (PCNA) labeling index,the bromodeoxyuridine (BrdU) labeling index and the serum gastrin were determined 3,5,7,10 and 14 days after reduced-size liver transplanta- tion.Results Hepatotoxieity of omeprazole has not been observed during the process of the experiments. Omeprazole significantly augmented MI,PCNA labeling index and BrdU labeling index of bepatocytes 5 days after transplantation (2.54±0.24)% vs (1.71±0.16)% (P<0.01),(26.96±2.09)% vs (18.73±1.94)% (P<0.01),(10.24±1.11)% vs (5.75±0.88)% (P<0.01) respectively.The ratio of remnant liver weight vs total liver weight and serum gastrin concentration in the experimental group were higher than those in control group one week after transplantation [(76.3±1.6)% vs (71.2±1.0)% (P<0.05),(441.9±25.9)ng/L vs (292.9±14.2) ng/L (P<0.05)] respectively and two weeks after transplantation [(94.5±1.7)% vs (86.9±1.5)% (P<0.01),(487.8±29.4) ng/L vs (291.7±21.6)ng/L (P<0.01)] respectively.Conclusion Omeprazole stimulates liver regeneration after reduced-size liver transplantation and this regeneration may be mediated by gastrin.更多还原