【摘要】 目的研究大鼠肝移植后树突状细胞(DC)的迁移情况。方法制作Wistar大鼠到SD大鼠的肝移植模型(实验组),并设Wistar大鼠间肝移植作为对照(对照组)。分别于术后第3、5、7天处死受者,切取移植肝组织及腹腔淋巴结,进行组织学观察,并以免疫组织化学染色观察移植肝组织和淋巴结中S-100~+DC的动态变化,以及CD25~+T淋巴细胞在淋巴结的活化增殖。结果肝组织病理学检查显示,实验组移植后第5天即出现轻至中度急性排斥反应。至第7天发展成中至重度排斥反应,受者存活时间(9.7±1.4)d。免疫组织化学染色显示,术后第3天实验组移植肝组织中DC数量明显增多,于第5天达到高峰,第7天则出现下降,明显高于对照组,差异有统计学意义(P<0.05)。从第3天开始,淋巴结中DC数量明显增多,第5、7天持续上升,与对照组相比,差异有统计学意义(P<0.05)。移植术后第3天,实验组淋巴结中CD25~+T淋巴细胞明显增多,呈现明显的T淋巴细胞活化增殖反应。结论同种异体肝移植后,DC对抗原的摄取和递呈加速,产生对T淋巴细胞强烈而持久的刺激,最终激活T淋巴细胞,导致排斥反应的发生。更多还原

【Abstract】 Objective To investigate migration of dendritic cells(DC)in orthotopic rat liver transplantation models.Methods Allogeneic models(Wistar→SD,experimental group)and syngenic models(Wistar→Wistar,control group)of rat liver transplantation were established.Graft livers and host celiac lymph nodes(n=4)of each group were sampled respectively at day 3,5,and 7 after the transplantation.The acute rejection was graded according to liver histopathological changes.The dy- namic state of DC number within graft and lymph nodes was detected by means of immunohistochemi- cal staining and image analysis.T-cell active proliferative response in lymph nodes was also studied. Results The histological examination revealed that mild to severe rejection occurred on the post-opera- tive days 5 and 7.At day 3 after transplantation,the number of S-100~+ DC in allograft was signifi- cantly increased and reached the peak at day 5,then decreased gradually at day 7.A significant num- ber of S-100~+ cells was detected in the allogeneic host lymph nodes from day 3 after transplantation, and displayed a continuous increasing trend for next several days.Active proliferation of T cells in the lymph nodes was triggered as early as day 3 after transplantation.Conclusion Allogeneic liver trans- plantation induces the accelerated migration of DC within the allograft and host lymphoid nodes.DC delivers a strong and sustained stimulation among T cells in the lymphoid nodes where effector cells are predominantly sensitized and rejected the graft eventually.更多还原