【摘要】 目的探讨DNA甲基转移酶(DNMT)的表达异常在SLE发病中的作用。方法以半定量RT-PCR方法检测了SLE缓解期、活动期及正常人外周血单核细胞(PBMC)中DNMT及CD11a基因表达水平,并进行相关性分析。结果SLE缓解期患者PBMC中DNMT1的表达水平显著低于正常人对照组(t=5.90,P<0.0001);活动期的表达水平也显著低于对照组(t=2.26,P=0.0001);缓解期与活动期比较差异无统计学意义(t=1.75,P=0.089)。SLE缓解期、活动期及对照组PBMC中DNMT3A的表达水平差异无统计学意义,DNMT3B的表达水平极低。SLE缓解期PBMC中CD11a表达水平明显高于对照组(t=5.35,P<0.0001);活动期的表达水平显著高于缓解期(t=2.99,P=0.006)和正常人对照组(t=6.57,P<0.0001)。DNMT1的降低与SLE疾病活动指数(SLEDAI)间无显著相关性(r=-0.34,P>0.05),CD11a的升高与SLEDAI间呈显著正相关(r=0.48,P<0.05),DNMT1与CD11a间无显著相关性(r=-0.18,P>0.05)。结论DNMT1表达水平降低在SLE的发病中可能起作用。但不是决定DNA甲基化状态的惟一因素。更多还原

【Abstract】 Objective To investigate the role of abnormal expression of DNA methyltransferases (DNMT1,DNMT3A,DNMT3B)and CD11a genes in the pathogenesis of systemic lupus erythematosus (SLE).Methods Semiquantitative reverse transcriptase-polymerase chain reaction was applied to detect the mRNA expression levels of DNMTs and CD11a in PBMCs of relieved(n=17),active(n=17)SLE patients and healthy controls(n=17).The correlations were further analyzed between these parameters. Results The expression of DNMT1 was significantly lower in PBMCs of both relieved and active SLE patients than that of health controls(t=5.90,P<0.0001;t=2.26,P=0.0001 respectively).No signifi- cant difference was observed in DNMT1 expression between relieved and active SLE patients(t=1.75,P= 0.089),or in the expression of DNMT3A in PBMCs between relieved,active SLE patients and healthy control.The expression of DNMT3B in PBMCs was extremely low.The expression of CD11a was signifi- cantly higher in relieved SLE patients than in the healthy controls(t=5.35,P<0.0001),in active SLE patients than in relieved SLE patients(t=2.99,P=0.006)and healthy controls(t=6.57,P<0.0001). No significant correlation was observed between the decrease of DNMT1 and SLE disease activity index (SLEDAI)(r=-0.34,P>0.05),or between the expression level of DNMTI and CD11a(r=-0.18, P>0.05).The increase of CD11a positively correlated with SLEDAI(r=0.48,P<0.05).Conclusion The decreased expression of DNMT1 may play an important role in the pathogenesis of SLE,but not the only factor contributing for DNA methylation status.更多还原