【摘要】 目的研究NET-1蛋白在皮肤鳞状细胞癌(SSCC)中表达的临床与病理意义。方法通过基因生物工程技术制备针对NET-1蛋白大部分细胞外区的多克隆抗体,应用免疫组化SP法检测NET-1基因蛋白在56例SSCC组织、50例皮肤上皮内瘤变(SINⅠ～Ⅲ级)及10例正常人皮肤(NS)组织中的表达。结果①NET-1蛋白阳性表达定位清晰,为细胞膜和/或胞质,组织背景非特异性反应弱或无;②NET-1仅表达在SINⅢ中上皮全层,阳性率为93.75%,在SSCC中表达阳性率为96.43%。NET-1在SSCC和SINⅢ中的阳性率显著高于SINⅠ～Ⅱ级,其阳性率为44.22%。③SSCC细胞不同Ki67表达强度、浸润深度和皮肤肿瘤TNM分期中NET-1表达除有显著筹异外,还与NET-1阳性表达呈显著正相关。在不同SSCC组织类型中,疣状鳞癌与梭形细胞鳞癌和经典鳞癌之间NET-1表达有显著差异,结论本文设计合成的NET-1抗体在SSCC石蜡标本中定位良好,可能是一个新的诊断SSCC的辅助组织学标记。更多还原

【Abstract】 Objective To investigate the expression,clinical and pathological significance of NET-1 protein in skin squamous cell carcinoma(SSCC).Methods The polyclonal antibody was prepared against the major extracellular domain of NET-1 protein by genetic engineering method.Skin tissue speci- mens were resected from 56 patients with SSCC,50 patients with skin intraepithelial neoplasia(SIN)Ⅰ-Ⅲand 10 normal controls,and all specimens were embeded in paraffin.Immunohistochemistry SP method was used to detect the NET-1 protein expression in these specimens.Results Immunohistochemically,NET-1 protein was clearly located in cell membrane and/or cytoplasm with a weak or absent nonspecific back- ground.The positive rates of NET-1 expression were higher in specimens of SINⅢand SSCC than in those of SINⅠand SINⅡ(93.75% and 96.43% vs 44.22%,both P<0.01).Significant difference was found in the positive rates of NET-1 expression among the specimens with different expression intensity of Ki67,with different tumor infiltration depth,at different TNM stage,and those collected from different histological types of SSCC(spindle-cell SSCC,verrucous SSCC and general SSCC).Furthermore,the expression intensity of NET-1 was positively correlated with that of Ki67,tumor infiltration depth and TNM stage. Conclusions The designed NET-1 antibody is well located in paraffin-embeded SSCC specimens,and might be a novel,complementary tissue marker for diagnosis of SSCC.更多还原