【摘要】 目的探讨γ-干扰素、全反式维A酸(ATRA)对皮肤鳞状细胞癌细胞株(SCL-1)表达生存素的影响。方法采用RT-PCR、免疫印迹(Western blot)分析方法分别检测γ-IFN、ATRA作用前后SCL- 1细胞株生存素mRNA及蛋白表达的差异。结果γ-IFN处理后,生存素在SCL-1细胞表达较对照组明显下降(P<0.05),且具有时间依赖性和剂量依赖性;ATRA可以增强γ-IFN对生存素的抑制作用。结论γ-IFN联合ATRA诱导皮肤鳞状细胞癌细胞发生细胞凋亡的机制之一可能与下调生存素的表达相关。更多还原

【Abstract】 Objective To study the effects of all-trans-retinoic acid（ATRA）andγ-interferon （γ-IFN）on the expression of survivin in skin squamous cell carcinoma cell line SCL-1.Methods SCL-1 cells were treated withγ-IFN alone（100,500,1000 u/mL,respectively）or in combination with ATRA (1000 u/mLγ-IFN + 10^-6mol/mL ATRA)in vitro.The mRNA and protein expressions of survivin were detected in SCL-1 cells by RT-PCR and Western-blot respectively.Resultsγ-IFN could significantly inhibit the mRNA and protein expressions of survivin in a dose-and time-dependent manner.Also,ATRA could enhance the inhibition of survivin expression byγ-IFN.Conclusion The down-regulation of survivin ex- pression may be a mechanism for the induction of apoptosis of skin squamous cell carcinoma cells by the combination ofγ-IFN and ATRA.更多还原