【摘要】 目的检测新疆维吾尔族寻常性银屑病(PsV)患者外周血DNA中HCR基因第16外显子的1911位点多态性,探讨其与银屑病发病的相关性。方法从128例患者及健康对照组132例的抗凝血中提取DNA,用PCR-限制性片段长度多态性法及PCR产物直接测序法鉴定基因类型,对结果进行统计学分析处理。结果HCR基因第16外显子的1911位点核苷酸存在G、A二态性,可表现为GG纯合、AA纯合、GA杂合三种基因型。基因型和等位基因频率经卡方检验两组比较,差异无统计学意义(P>0.05)。其中Ⅰ型、Ⅱ型寻常性银屑病患者与对照组比较,差异无统计学意义(P>0.05),男女寻常性银屑病患者与对照组比较,差异无统计学意义(P>0.05)。结论HCR基因第16外显子1911位点的多态性与新疆维吾尔族非家系寻常性银屑病在基因型和等位基因频率方面未发现相关性。更多还原

【Abstract】 Objective To detect the polymorphism of HCR gene,and to investigate the relationship of it with psoriasis vulgaris in Uigur population of Xinjiang Uigur Autonomous Region,China.Methods Blood samples were collected from 128 patients with psoriasis vulgaris and 132 healthy controls.DNA was extracted from the samples and subjected to test with PCR.Genotypes were identified with restriction frag- ment length polymorphism(PCR-RFLP)and gene sequencing.The genotype frequency was compared be- tween the patient and control groups.Results A G/A polymorphism,including three genotypes of GG,GA and AA,was identified at position 1911 in exon 16 of HCR gene.There was no significant difference in genotype distribution or allele frequency in this site between the patient and control groups(P>0.05). Similarly,no significant difference was found in them when the patients of TypeⅠandⅡwere compared with the controls,respectively,or when male and female patients were compared with the controls,respec- tively.Conclusion No correlation is found between the polymorphism at position 1911 in exon 16 of HCR gene and development of non-familial psoriasis vulgaris in Uigur population of Xinjiang Uigur Autonomous Region.更多还原