【摘要】 目的通过观测小剂量超短波(USW)对大鼠脑缺血再灌注后脑梗死体积,脑组织中B细胞淋巴细胞瘤-xl(Bcl-xl)及肿瘤坏死因子-α(TNF-α)表达的影响,进一步探讨小剂量USW对脑缺血再灌注损伤保护作用的机制。方法用线栓法制备一侧大脑中动脉栓塞再灌注大鼠模型,造成大鼠右脑缺血2h再灌注24h,采用Longa 5级评分法评定神经功能缺损程度。大鼠按体重随机分成空白对照组、对照组及超短波治疗组(USW组),后2组选择Longa 5级评分法为2分的大鼠。所选大鼠均于再灌注24h后断头取脑。分别测定脑梗死灶体积、脑组织中Bcl-xl及TNF-α的表达。结果与对照组比较,USW组梗死体积及梗死体积占全脑体积的百分比均降低,差异具有统计学意义(P<0.05)。与对照组比较,USW组脑组织中Bcl-xl表达增加,TNF-α水平降低,差异均具有统计学意义(P<0.05)。结论小剂量超短波可通过增加Bcl-xl表达,降低TNF-α水平而抑制脑神经细胞凋亡,挽救半暗带,缩小脑梗死灶,减小梗死体积,从而保护脑缺血再灌注后损伤神经,进而提高治疗效果。更多还原

【Abstract】 Objective To observe the effect of low dosage of uhrashortwave(USW) on infarction volume, B cell lymphocytoma-xl (Bcl-xl) and tumor necrosis factor alpha (TNF-α) after cerebral ischemia-reperfusion in rats and discuss its acting mechanisms. Methods Focal ischemia-reperfusion model was established in 25 rats by re- versible right middle cerebral artery occlusion with filament. The right side cerebral ischemia was lasted for 2 hours and then followed with 24 hours of reperfusion. The content of neurological deficits were evaluated by the Zea-Longa 5-degree scoring system to select rats. After surgery, the rats were divided into 3 groups: blank control group, control group and USW treatment group. The brain of all rats was taken at 24 hours after reperfusion. The cerebral infarction volume, the expression of Bcl-xl and TNF-αwere measured and analyzed. Results Twenty-five rats were used in the analysis of results. When compared with the control group, the infarction volume and rate in total cerebral volume of USW group significantly decreased (t = 2.54, 2.33, P<0.05 ), the exprssion of Bcl-xl significantly increased, and the expression of TNF-αdecreased(P<0.05). Conclusion Low dosage of USW therapy can protect reperfu- sion injuries after cerebral ischemia and increase the therapeutic effect by increasing the expression of Bcl-xl and de- creasing the level of TNF-αto inhibit neuron apoptosis, save ischemic penumbra and decrease infarction volume.更多还原