【摘要】 目的探讨武汉市美沙酮门诊治疗者丙型肝炎病毒(HCV)及不同基因型感染状况。方法用酶标记免疫(ELISA)法检测抗-HCV抗体,在86份抗-HCV抗体阳性的血清标本中,分别提取HCV RNA,通过逆转录巢式聚合酶链反应(RT-nested PCR)扩增C基因的羧基至E1基因的氨基端长度为474 bp的片段,测定其核苷酸序列,与GenBank中已知的HCV序列进行系谱分析,确定HCV基因型。结果武汉市美沙酮门诊332名治疗者中抗-HCV IgG阳性率为94.3%;其中86份血清的HCV序列通过系谱显示6a型71例,占82.5%;3b 7例,占8.2%;1a 5例,占5.8%;1b 3例,占3.5%。结论武汉市美沙酮门诊入组的吸毒者HCV感染以6a型为优势株,其次为3b,吸毒者中HCV感染率较高,且基因亚型呈现多样性。更多还原

【Abstract】 Objective To study the prevalence of hepatitis C virus (HCV) infection and characteristics on molecular biology related to HCV among patients who were enrolled in a Methadone maintenance clinic in Wuhan.Methods Serum samples from 332 injection drug users (IDUs) were obtained and anti-HCV IgG was detected by enzyme linked immunosorbrent assay(ELISA),together with 86 anti-HCV positive specimens genotyped.A reverse transcriptase-polymerase chain reaction (RT-nPCR) assay using conserved primers deduced from the core-envelopel (C-E1) region of the HCV genome was employed to amplify a 474 bp fragment.Phylogenetic analysis of the C-E1 sequences was conducted by direct sequencing of the RT-nPCR products and alignment with determined by nucleotide sequencing followed by composition of a phylogenetic tree.Results There were 313 cases (94.3%) appeared positive anti-HCV IgG in the 332 patients from a Methadone maintenance clinic in Wuhan.It was demonstrated that there were four different subtypes of HCV in that clinic in Wuhan,including 6a--71 cases (82.5%), 3b--7 cases (8.2%),1a--5 cases (5.8%) and 1b--3 cases (3.5%).Conclusion Infection of 6a genotype HCV was predominant in patients from the Methadone maintenance clinic in Wuhan,followed by HCV 3b,1a and 1b.更多还原