【摘要】 目的应用血型糖蛋白A(GPA)基因突变频率等指标探寻影响苯作业工人GPA基因突变频率的相关因素,探讨该指标用于预测苯中毒风险的可行性。方法采用固定群体随机抽样的方法,选取慢性苯中毒工人40例及其对照82例,分离、固定外周血红细胞,与荧光素标记的单抗结合后,采用流式细胞术进行GPA突变分析;应用统计学方法对影响GPA基因突变频率的相关因素进行分析。结果慢性苯中毒组GPA基因突变频率明显高于对照组GPA基因突变频率,差异有统计学意义(P<0.05)。年龄、工龄、苯接触累积评分与苯作业工人GPA基因突变频率呈正相关关系(P<0.01),3-氨基苯甲酰胺(3AB)指数与GPA基因突变频率之间呈负相关(GPANO:r_s=-0.589,P<0.01;GPANN:r_s= -0.615,P<0.01)。多因素回归分析,苯接触和个体易感性因素进入模型,决定系数R~2=0.819。结论苯接触和个体易感性因素是影响GPA基因突变频率的重要因素。苯接触水平越高,GPA基因突变频率越高;个体的易感性越强,GPA基因突变频率越高。更多还原

【Abstract】 Objective To analyze related factors which affect GPA mutation frequency of workers ex- posed to benzene,with the Glycophorin A(GPA)mutation assay and explore the possibility of GPA mutation frequency as an index of predicting the risk of benzene poisoning.Methods The erythrocytes were bound with fluorescent-labeled monoclonal antibody after isolated and fixed from the peripheral blood,and then the GPA mutation assay was performed using the flow cytometry(FCM).The related factors of GPA mutation fre- quency were analyzed by statistical methods.Results The GPA mutation frequency of chronic benzene poi- sonings was significantly higher than that of their controls(P<0.05).Significant direct correlation was found be- tween age,length of service,accumulative exposure score and the GPA mutation frequency of workers exposed to benzene(P<0.01).However,there was significantly inverse correlation between the 3AB index and the GPA mutation frequeney(GPAN0:r_s=-0.589,P<0.01;GPANN:r_s=-0.615,P<0.01).In the multiple factor regression analysis on GPA mutation frequency,benzene exposure and individual susceptibility both entered model of muhiple factors analysis,the coefficient of determination of benzene-exposed workers was 0.819.Conclusion Exposure to benzene and individual susceptibility are the most important factors that affect GPA mutation fre- quency.GPA mutation frequency increases with the benzene exposure and individual susceptibility.更多还原