【摘要】 目的观察在百草枯(PQ)致大鼠肺纤维化模型中肺组织胎盘生长因子(PLGF)的变化。方法将42只健康成年雌性SD大鼠随机分为对照组和染毒组,每组21只,分别观察7、14和28 d。染毒组于实验开始一次性灌胃染毒PQ(40 mg/kg),对照组予以等量的生理盐水。取肺组织行半定量的病理组织学检查、羟脯氨酸测定,并利用半定量逆转录-聚合酶链反应(RT-PCR)和免疫组化方法测定PLGF mRNA和蛋白表达。结果PQ染毒后大鼠肺泡炎、肺纤维化程度积分明显增高,肺组织羟脯氨酸含量明显高于对照组,差异有统计学意义(P<0.01)。7、14、28 d时肺组织PLGF mRNA表达分别1.28±0.29、0.80±0.07、0.65±0.13,均高于对照组,差异有统计学意义(P<0.01);免疫组化阳性指数在3个时点分别为2.27±0.34、1.78±0.41、1.25±0.69,明显高于对照组,差异有统计学意义(P<0.01)。结论PQ中毒大鼠肺组织PLGF mRNA和蛋白水平的表达明显上调。更多还原

【Abstract】 Objective To investigate the dynamic expression of placenta growth factor（PLGF）in the lungs with paraquat（PQ）-induced pulmonary fibrosis.Methods Forty-two adult healthy female Sprague-Daw- ley（SD）rats were randomly divided into two groups:the control group and the PQ group.Each group was divid- ed into three subgroups,seven animals each.The rats in PQ group were treated intragastrically（ig）with PQ（40 mg/kg）and the rats in control group were treated with the same volume of saline at the beginning of the experi- ment.The animals of model and control group were sacrificed and lungs were harvested on the 7^th,14^thand 28th days respectively.A semiquantitative assay of histological examination and hydroxyproline in lung tissues were used to determine the severity of alveolitis and fibrosis.RT-PCR and immunohistochemistry were used to detect the mRNA and protein expression of PLGF.Results Hydroxyproline contents in lung tissue were significantly increased after PQ administration.Inflammatory cell infiltration and fibrotic scores were more prominent in the model group compared to the control group.Further study showed that PLGF mRNA on day 7,14 and 28（1.28±0.29,0.80±0.07,0.65±0.13）and positive index of protein expression（2.27±0.34,1.78±0.41,1.25±0.69）in the PQ group were all upregulated as compared with those of the control group.Conclusion The PLGF expression in the lung tissue in rats with paraquat-induced pulmonary fibrosis is upregulated.更多还原