【摘要】 目的比较在体和离体2种测量方法测定氯丙烯(AC)亚慢性中毒大鼠坐骨神经的电生理指标的改变。方法90只Wistar雄性大鼠随机分为对照组(n=50)和染毒组(n=40),染毒组大鼠以200mg/kg剂量AC灌胃,每周3次,在染毒3、6、9、12周,分别采用在体和离体2种方法测定相应对照组和染毒组大鼠坐骨神经传导速度(NCV)、复合动作电位峰值(CAPA)、潜伏期(PL)、时程(TC)、阈电位(TP)及最大刺激电位(MSP)等指标。结果与对照组相比,在染毒6、9和12周,NCV在体测定分别降低23.6%、40.4%和48.6%,离体测定染毒12周,NCV降低15.4%,差异均有统计学意义(P<0.05)。染毒前9周,在体测定CAPA无明显变化,染毒第12周,与对照组相比下降了31.7%,染毒6、9、12周,离体测定CAPA与对照组比较分别降低31.7%、38.9%和58.9%,差异均有统计学意义(P<0.05,P<0.01)。PL在体测定第9、12周增加22.6%和40.7%,离体测定第12周增加8.0%,差异均有统计学意义(P<0.01,P<0.05);TC在体测定第6、9、12周分别增加22.5%、34.6%和47.5%,离体测定第12周增加19.5%,差异均有统计学意义(P<0.01);第9、12周,TP在体测定分别增加32.3%和40.0%,离体测定分别增加29.2%和35.6%,差异有统计学意义(P<0.05);MSP在体测定分别增加69.0%和86.5%,离体测定分别增加52.0%和61.9%,差异均有统计学意义(P<0.01)。结论2种测定方法均表明AC可明显改变大鼠坐骨神经电生理特性,并且具有明显的时间依赖性,但其测定的敏感指标不同。在体测定NCV是最敏感指标;离体测定CAPA是最敏感指标,可为AC中毒性神经病早期诊断提供依据。更多还原

【Abstract】 Objective To compare the results of in vivo and in vitro in determination of the changes of allyl chloride(AC)-induced electrophysiology in rats sciatic nerve.Methods Ninety male Wistar rats weight- ed 180～220 g were divided randomly into two groups,i.e.experimental group(n=40)and control group(n=50). The rats in experimental group were treated with AC dissolved in corn oil(200 mg/kg ip 3 days/week)by gav- age for 12 weeks.Electrophysiological indexes of each group were determined on 3,6,9 and 12 weeks of AC intoxication.The indexes included measurements of sciatic nerve conduct velocity(NCV),compound action po- tential amplitude(CAPA),potential latency(PL),time course(TC),threshold potential(TP)and max stimulate potential(MSP).Results Compared to the corresponding time-matched control rats,on 6,9 and 12 weeks of AC intoxication,NCV were decreased by 23.6%,40.4% and 48.6%(P<0.05,P<0.01)in vivo,while in vitro it was decreased by 15.4%(P<0.05)on 12 week,CAPA were reduced by 31.7% in vivo,while in vitro it was re- duced by 31.7%,38.9% and 58.9%(P<0.05,P<0.01),respectively;PL were prolonged 22.6% and 40.7%(P<0.01)on 9,12 weeks in vivo,while in vitro it was prolonged 8.0%(P<0.05),TC were increased 22.5%,34.6% and 47.5%(P<0.01)in vivo,while in vitro it was increased 11,6%,20.0%(P>0.05)and 19.5%(P<0.01),re- spectively;TP were elevated 12.1%(P>0.05),32.3% and 40.0%(P<0.05)in vivo,while in vitro it was elevated 16.4%(P>0.05),29.2% and 35.6%(P<0.05),respectively;MSP were increased 40.5%(P<0.05),69.0% and 86.5%(P<0.01)in vivo,while in vitro it was increased 29.7%(P>0.05),52.0% and 61.9%(P<0.01),respec- tively.Conclusion The two methods of in vivo and in vitro showed that AC could significantly affect the elec- trophysiology of sciatic nerve,and the time-dependent changes occurred.The NCV is the most sensitive indica- tor in vivo to the early diagnosis of AC intoxication,while CAPA is the most sensitive indicator in vitro.更多还原