【摘要】 目的初步探索职业性慢性铅中毒患者T淋巴细胞亚群及Th淋巴细胞因子的变化特点。方法对23例职业性慢性铅中毒患者（铅中毒组）及20例健康非职业铅接触成人（对照组）采用流式细胞技术检测外周静脉血淋巴细胞CD₃、CD₄、CD₈的表达,同时通过流式细胞微球芯片捕获技术检测血浆IL-2、IL-4、IL-6、IL-10、TNF-αt、IFN-γ的浓度。结果铅中毒患者外周静脉血CD₄相对百分比（32.68%±11.54%）及CD₄/CD₈比值（0.89±0.39）与对照组比较,明显下降,差异有统计学意义（P<0.05）;血浆IL-2浓度为（2.00±0.68）pg/ml,与对照组比较明显上升,差异有统计学意义（P<0.05）;血浆IL-10和IFN-γ浓度分别为（1.83±0.85）pg/ml和（3.42±0.85）pg/ml,与对照组比较明显减低,差异有统计学意义（P<0.05）。结论T淋巴细胞亚群及细胞因子对评价铅免疫毒性有进一步研究价值。更多还原

【Abstract】 Objective To investigate the changes in T lymphocyte subsets and plasma Th1/Th2 cy- tokine levels in patients with occupational chronic lead poisoning.Methods Flow cytometric analysis was used to count the numbers of CD3~+,CD4~+,CD8~+ cells and cytometric bead array (CBA) capture technique was used to quantify plasma IL-2,IL-4,IL-6,IL-10,TNF-α,IFN-γconcentrations.Twenty-three patients with oc- cupational chronic lead poisoning and 20 controls were examined.Results Compared with control group, CD3~+ CD4~+ lymphocytes and CD4/CD8 ratios decreased in patients with occupational chronic lead poisoning. The plasma IL-2 level were significantly raised while the IL-10 and IFN-γwere reduced in patients with occu- pational chronic lead poisoning.Conclusions T lymphocyte subsets and plasma Th1/Th2 cytokine levels could be a new parameter to assess lead immunotoxity.更多还原