【摘要】 目的研究黄芩茎叶总黄酮(TF)对人类单核肿瘤细胞U937增殖的影响。方法U937细胞以5×10~4个/ml的浓度,培养于含胎牛血清(FCS)10%的RPMI 1640全培养液中,TF浓度分别为0、25、50、100、150、200 mg/L组,每组8孔接种于24孔培养板中孵育7 d。每天计数各孔细胞密度,第7天测定细胞悬液中乳酸脱氢酶(LDH)活性。结果TF 25～200 mg/L组U937细胞密度均低于TF 0 mg/L组,浓度越高细胞密度越低(P<0.05)。TF 0～150 mg/L组细胞悬液LDL活性差别无统计学意义(P>0.05),TF 200 mg/L组LDL与其他5组相比均具有统计学意义(均P<0.05)。结论TF具有抑制U937细胞增殖的作用,高浓度的TF(≥200 mg/L)对体外培养的U937细胞具有毒性作用。更多还原

【Abstract】 Objective To study the effects of total flavone of scutellarla’s stem and leaf(TF)on U937 cell proliferation.Methods 48 holes of U937 cells(5×10~4/ml)cultured in RPMI1640 with 10% fetus cattle serum (FCS)were divided into 6 groups according to the concentration of TF(0 mg/L,25 mg/L,50 mg/L,100 mg/L,150 mg/L,200 mg/L).Every group was incubated for 7 days.Cell counting was applied every day and the activity of lac- tate dehydrogenase(LDH)in culture medium was measured on the 7th day.Results The density of U937 cells in TF 25～200 mg/L groups was significantly lower than that in TF 0 mg/L group(P<0.05).The activity of LDH in TF 0～150 mg/L group had no significant difference(P>0.05),and that in 200 mg/L group was higher than other groups(P<0.05).Conclusion TF inhibits proliferation of U937 cells.TF probably poisons U937 cells with the concentration higher than 200 mg/L.更多还原