【摘要】 目的筛选与肺癌转移相关的标志分子。方法利用单向变性凝胶电泳(SDS-PAGE)和基质解吸附时间飞行质谱(MALDI-TOF-MS)对具有不同转移能力的肺鳞癌株 NCI-H226及其脑转移亚系 H226Br 的培养基上清进行分析,鉴定出12种标志蛋白,其中乳酸脱氢酶 B 链(L-lactatedehydrogenase B,LDHB)表达水平明显升高。利用酶联免疫吸附技术(ELISA)对105例肺癌、30例喉癌、41例肺炎和肺结核患者及65名健康人血清中 LDHB 的表达进行分析。结果与肺炎和肺结核患者[A 值为0.187(0～0.609),P<0.01]及健康对照组[A 值为0.159(0～0.524),P<0.01]相比,肺癌患者 LDHB 水平[A 值为0.485(0～1.415)]明显升高,且随病程发展呈现出递增的趋势。在操作特异性曲线(receiver operating characteristic,ROC)临界值取为0.260时,敏感性为81%,特异性为70%,总体精确性为76%。结论分泌蛋白质组学可以成为一种寻找与癌症发生和发展相关的新标志蛋白的有效方法。更多还原

【Abstract】 Objective To identify potential biomarkers related with lung cancer metastasis. Methods Conditional media proteins collected from a primary non-small cell lung cancer cell line (NSCLC)NCI-H226 and its brain metastatic subline H226Br were analyzed by SDS-PAGE and MALDI- TOF-MS.Twelve biomarkers were identified,of which LDHB was significantly up-regulated in H226Br cell and was further validated using ELISA in sera including 105 lung cancer samples,41 pneumonia and pulmonary tuberculosis samples and 65 healthy samples.Results The levels of LDHB were specifically elevated in NSCLC sera [ A value 0.485(0-1.415)] compared with pneumonia and pulmonary tuberculosis [A value 0.187(0-0.609),P<0.01]and healthy group [A value,0.159(0-0.524),P< 0.01 ] and were progressively increased with the clinical stage.At the cutoff point 0.260(A value)on the ROC curve,the sensitivity,specificity and total accuracy of LDHB were 81% ,70% and 76% respectively. Conclusion These findings demonstrated that secretome could open up a possibility to identify novel biomarkers related with cancer occurrence and metastasis.更多还原