【摘要】 目的探讨儿茶酚-O-甲基转移酶（COMT）基因多态性与子宫内膜癌遗传易感性的关系。方法以聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法,对132例子宫内膜癌患者（内膜癌组）和110例子宫脱垂、宫颈鳞癌和卵巢良性囊肿患者（对照组）的 COMT 基因第4号外显子第158位密码子 G 与 A 的多态性进行分析,用非条件 logistic 回归分析 COMT 基因多态性与子宫内膜癌发生风险的关系。结果对照组患者以 COMT^Val/Val基因型为主（47.2%,52/110）,COMT^Val/Met（45.5%,50/110）和 COMT^Met/Met（7.3%,8/110）次之;内膜癌组则以 COMT^Val/Met基因型为主（58.3%,77/132）,COMT^Val/Val（29.5%,39/132）和 COMT^Met/Met（12.1%,16/132）次之,两组比较,差异有统计学意义（P<0.05）。经非条件 logistic 回归分析,与 COMT^Met/Met基因型相比,COMT^Val/Val基因型者的子宫内膜癌发生风险的 OR 值为0.262（95% CI 为0.080～0.862,P=0.027）。结论中国南方汉族妇女的 COMT 基因型以 COMT^Val/Val最多,携带 COMT^Val/Val基因型的妇女相对于 COMT^Met/Met基因型者,其发生子宫内膜癌的风险可能降低。更多还原

【Abstract】 Objective The 4-and 16-hydroxylated metabolites of estrogens have been implicated in carcinogenesis,whereas its 2-hydroxylated metabolites have been shown to have antiangiogenic effects.We aimed to examine whether the polymorphisms of catechol-O-methyltransferase（COMT）involved in the estrogen metabolism are associated with endometrial cancer risk.Methods Polymerase chain reaction- restrictive fragment length polymorphism（PCR-RFLP）analysis was used to study the variant allele frequency distributions of COMT Val158Met genetic polymorphism in a population based case-control study with 132 endometrial cancer cases and 110 controls.Odds ratios（OR）and 95% confidence intervals（CI） were estimated by unconditional logistic regression after adjustment for known or suspected risk factors for endometrial cancer.Results The most frequent genotype was COMT^Val/Val（47.2%,52/110）in control group and COMT^Mal/Met（58.3%,77/132）in endometrial cancer group.The difference between the two groups was of statistical significance（P<0.05）.Compared with COMT^Met/Metgenotype,the COMT^Val/Val genotype was inversely correlated with endometrial cancer risk,and the adjusted OR value was 0.262（95% CI:0.080-0.862,P=0.027）.Conclusions Among the genotypes in women in South China,genotype COMT^Val/Valis mostly seen,followed by COMT^Val/Met,and COMT^Met/Metis the least in control group.The endometrial cancer susceptivity of genotype COMT^Val/Valcarriers may be lower than COMT^Met/Metcarriers.更多还原