【摘要】 目的研究乙肝病毒 X 蛋白(HBx)上调的基因着丝粒蛋白 A(centromere protein A,CENP-A)在原发性肝细胞癌(HCC)及癌旁组织的表达差异,并初步探讨其意义。方法应用 RT-PCR 和即时定量 PCR(real-time quantitative PCR)方法,检测20例 HCC 及配对癌旁肝组织中 CENP-A基因 mRNA 的表达水平,应用免疫组织化学方法检测80例 HCC 和癌旁肝组织中 CENP-A 蛋白、p53蛋白的表达情况。结果 CENP-A mRNA 在癌及癌旁组织中的相对表达水平分别为(0.64±0.18)和(0.09±0.09),二者差异有统计学意义(t=12.78,P<0.01)。免疫组织化学结果显示:CENP-A 在HCC 组织中的总阳性率为71.25%(57/80),高于配对癌旁组织的总阳性率43.75%(35/80)(χ~2=12.38,P<0.01)。p53在癌组织中的总阳性率为75%(60/80),高于配对癌旁组织中的总阳性率16.25%(13/80)(χ~2=55.65,P<0.01),p53与 CENP-A 在癌组织中的表达一致性达88.75%(71/80),呈正相关(r=0.57,P<0.01)。结论 CENP-A 在肝癌组织中的过表达与细胞恶性增殖相关,且这种过表达是由转录水平引起的,其机制可能与 p53基因的转录调节有关。更多还原

【Abstract】 Objective To study the expression of centromere protein A(CENP-A)and its significance in hepatocellular carcinoma(HCC)and adjacent non-neoplastic liver tissue.Methods The expression levels of CENP-A mRNA in 20 samples of HCC and adjacent non-neoplastic liver tissue were analyzed by reverse transcription-polymerase chain reaction(RT-PCR)and real-time quantitative polymerase chain reaction(qRT-PCR).Immunohistochemical study for CENP-A and p53 proteins was also performed on tissue microarrays containing 80 samples of HCC and adjacent liver tissue.Results The expression level of CENP-A mRNA in HCC(0.64±0.18)was higher than that in adjacent non-neoplastic liver tissue (0.09±0.09)(t=12.78,P<0.01).Of the 80 samples of HCC,57 cases(71.25%)and 60 cases (75%)expressed CENP-A and p53 proteins respectively.The positivity rates of CENP-A and p53 proteins in non-neoplastic liver tissue were 43.75%(35/80)and 16.25%(13/80)respectively.There was a statistically significant difference in CENP-A and p53 protein expression between HCC and non-neoplastic liver tissue(P<0.01).The coincident rate between CENP-A and p53 expression was 88.75%(71/80). Expression of CENP-A protein showed a positive correlation with that of p53 protein(r=0.57,P<0.01). Conclusion The over-expression of CENP-A occurs at transcriptional level and may be related to malignant proliferation of HCC via possible interaction with p53 gene.更多还原