【摘要】 目的:建立同时测定彝肤软膏中重楼皂苷Ⅰ、重楼皂苷Ⅱ高效液相色谱分析方法。方法:采用高效液相色谱法对彝肤软膏中重楼皂苷Ⅰ、重楼皂苷Ⅱ同时进行含量测定:Hypersil ODS2(4.6 mm×150 mm,5μm);预柱:IBBM-612111,C-18柱芯;乙腈-0.02%磷酸(43∶57)为流动相,流速1 mL.min-1,检测波长203 nm,柱温40℃。结果:重楼皂苷Ⅰ在0.102 4~3.2μg线形关系良好,r=0.9998,平均回收率97.4%,RSD 1.8%(n=6);重楼皂苷Ⅱ在0.064~2.0μg线形关系良好,r=0.9999,平均回收率101.4%,RSD 1.0%(n=6)。结论:本方法准确可靠、重复性好,能有效控制该制剂的质量。更多还原

【Abstract】 Objective: To develop a method for the determination of Chonglou saponin Ⅰand Chonglou saponin Ⅱ in Yifu ointment.Method: The chromatographic separation was performed on Hypersil ODS2 C18 column(4.6 mm×150 mm,5 μm) and IBBM-612111 ODS C18 guard column.Acetonitrile-0.02% phosphoric(43∶57) as mobile phase.The flow rate was 1 mL·min-1 and column temperature was set at 40 ℃.The UV detection wavelength was set 203 nm.Result: Chonglou saponinⅠshowed a good linear relationship at a range of 0.102 4-3.2 μg,r=0.999 8,the average recovery was 97.4%,and RSD was 1.8%(n=6);Chonglou saponin Ⅱ showed a good linear relationship at a range of 0.064-2.0 μg,r=0.999 9,the average recovery was 101.4%,and RSD was 1.0%(n=6).Conclusion: The method is accurate with the good reproducibility and can be used for the quality control of Yifu Ointment.更多还原