【摘要】 目的:对青蒿P450 cDNA基因进行克隆和生物信息学分析。方法:通过RT-PCR,从青蒿cDNA中克隆了1个P450基因;通过生物信息学方法,对其结构特点进行分析。结果:该P450 cDNA基因ORF为1 464 bp,编码488个氨基酸,该序列在GenBank上的登录号为DQ667171。编码蛋白是一个中等大小的A型P450,相对分子质量54.992 kDa;等电点8.83,定位于线粒体,和CYP71AV1具有很高的同源性。结论:该青蒿P450和CYP71AV1在进化和性质上很接近,可能在青蒿中执行类似功能。更多还原

【Abstract】 Objective: Cloning and bioinformatics analysis of P450 cDNA in Artemisia annua.Method: A P450 cDNA gene was cloned from A.annua by RT-PCR.The bioinformatics analysis of the P450 gene was performed.Result: The complete ORF of this P450 cDNA is 1 464 bp and encodes 488 aa.The sequence was reported to GenBank and coded as DQ667171.Bioinformatics analysis of the P450 cDNA showed it encoded an A-type P450 protein with 54.992 kDa,it’s isoelectric point was 8.83 and the possibility of export to mitochondria was 0.893 2.Conclusion: The comparable analysis of the P450 with CYP71AV1 revealed that the two proteins probably performed the same function because of the similar character.更多还原