【摘要】 目的:研究超临界CO2萃取技术提取鹿茸中性激素。方法:超临界CO2萃取鹿茸中的性激素并采用放射免疫法分析其含量,利用GC-MS,TLC和HPLC分析了萃取物中其他成分。结果:以85%乙醇为夹带剂,夹带剂∶原料=1(mL.g-1),萃取压力30 MPa,萃取温度65℃,总提取率为1.56%,雌二醇和孕酮的质量分数分别为3.07,776.18 ng.g-1。结论:采用超临界CO2萃取技术提取鹿茸中的性激素是可行的。更多还原

【Abstract】 Objective: To study the method of extraction of sex hormone from antler velvet with supercritical CO2.Method: Supercritical fluid extraction(SFE) was used to extract sex hormone from antler velvet and radioimmunoassay(RIA) was used to analyze the extracts.The chemical compositions in extracts were identified by GC-MS,TLC and HPLC,respectively.Result: The experimental results indicated that the extraction yield was 1.56% when 85% ethanol was used as co-solvent at temperature of 65 ℃ and extraction pressure of 30 MPa.Estradiol and progesterone in the extracts were 3.07,776.18 ng·g-1 respectively.Conclusion: It is feasible to extract hormone from antler velvet with supercritical CO2.更多还原