【摘要】 目的观察重组人甲状旁腺素(1-34)与雌激素单用和联用对去卵巢大鼠骨代谢的影响。方法选用雌性4月龄SD大鼠45只,随机分为5组:①假手术(Sham)8只;②去卵巢(OVX)9只;③雌激素治疗组(OVX+E)10只:OVX2个月后给予苯甲酸雌二醇治疗6w;④PTH治疗组(OVX+PTH)9只:OVX2个月后给予rhPTH(1-34)治疗6w;⑤雌激素与PTH联合治疗组(OVX+E+PTH)9只:OVX2个月后给予rhPTH(1-34)和E2联合治疗6w。观察各组大鼠胫骨近端松质骨骨小梁形态计量学参数,椎体生物力学指标及部分血清骨生化代谢指标。结果雌激素治疗组和PTH治疗组的骨静态参数均表现为骨量增加;雌激素治疗组的骨形成参数和骨吸收参数降低;PTH治疗组的骨形成指标明显升高,而骨吸收指标虽较Sham高,但较OVX组有所降低;E2与PTH联合治疗组与单用E2组和单用PTH组比较,骨量明显提高,骨转换率参数介于单用E2和单用PTH组之间。3个治疗组的生物力学指标较OVX组均有明显提高,其中E2与PTH联合治疗组改善最明显。结论雌激素和PTH可使去卵巢大鼠松质骨骨量增加和改善生物力学性能,两者联合治疗有协同作用。更多还原

【Abstract】 Objective To investigate the effects of rhPTH（1-34） and estrogen on bone metabolism in ovarictomized rats. Methods Forty five female Sprague-Dawley rats, four months old, were divided randomly into 5 groups: Sham-operated （Sham, 8）, ovariectomized group （OVX, 9）, ovariectomized and treated with estrogen （OVX+E, 10）, ovariectomized and treated with parathyroid hormone （OVX+PTH, 9）, ovariectomized and treated with estrogen and parathyroid hormone （OVX+E+PTH, 9）. After three and half months, all animals were sacrificed. Static and dynamic parameters of right tibias, biomechanics parameters and some related bone metabolic markers are evaluated. Results Both E₂ and PTH treatment increase bone mass. The parameters of formation and resorption decreased in E group while the bone formation rate increased in OVX+PTH group in comparison to OVX. Parameters of absorption of OVX+PTH group was higher than that of Sham group, but lower than OVX group. Parameters of bone mass and formation in rats treated with PTH+E revealed a significant increase not only compared with the OVX+E group, but also compared with OVX+PTH group. The parameters of bone turnover in OVX+PTH+E group between the PTH and E₂ individual treatment. The biomechanics parameters in E, PTH and E+PTH treated rats were slightly elevated compared to OVX rats, especially the OVX+E+PTH group. Conclusions Estrogen and PTH increases bone mass and biomechanics parameters effectively in ovariectomized rats. The combination treatment of PTH and Estrogen resulted in a marked overall increase of cancellous bone and exhibited synergistic effects.更多还原