【摘要】 目的:建立高效液相测定人工培养蛹虫草子实体中核苷类成分含量的方法。方法:采用KNAUER Eurospher-100 C18色谱柱(4.6mm×250mm,5μm),以乙腈-50mmol.L-1磷酸二氢钾(7∶93)为流动相,检测波长为260nm。结果:测定3批人工培育蛹虫草中核苷类成分的含量,腺苷、腺嘌呤、虫草素的平均含量分别为0.14%,0.0095%,0.42%。结论:本方法能同时测定人工蛹虫草中多种核苷类成分的含量,具有较高的灵敏度,操作简单,可用于人工蛹虫草的质量评价。更多还原

【Abstract】 OBJECTIVE To develop a method for the determination of adenosine,adenine and 3’-deoxyadenosine in cultured Cordyceps militaris L.Link by HPLC.METHODS A KNAUER Eurospher-100 C18 column was used with acetonitrile-50 mmol·L-1 KH2PO4(7∶93)as the mobile phase and the detection wavelength was 260 nm.RESULTS The average contents of adenosine,adenine and 3’-deoxyadenosine were 0.14%,0.009 5% and 0.42%.CONCLUSION This method is sensitive,rapid and accurate with good reproducibility for quantitative analysis of nucleosides in cultured Cordyceps militaris L.Link.更多还原