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降解除草剂异噁草酮细菌的分离、鉴定及生长特性
Studies on screening,identification and growth characters of the herbicide clomazone degrading-bacteria
【摘要】 利用长期施用长残留除草剂的土壤,采用高压富集的方法,从两种培养液中共分离出3株细菌,根据菌株的形态特性和生理生化特征,对此3个菌株进行了初步鉴定:W2为短杆菌属(Brevibacterium);Y1和X同为芽胞杆菌属(Bacillus);W2、Y1和X的最适生长温度分别是30℃、35℃和30℃;W2培养基的pH值为6.0~9.0,Y1和X的最适pH值为6.0~8.0;W2生长最适异噁草酮的浓度是200mg a.i..L-1,Y1和X生长最适异噁草酮的浓度是100mg a..i..L-1。
【Abstract】 Three bacteria strains capable of degrading herbicide clomazone in 2 basal liquid meadia were isolated from soil,polluted by the pesticide,using batch culture with clomazone as a sole carbon.W2 was identified as Brevibacterium,Y1 and X were identified as Bacillus sp.,based on the morphological appearances and physiological characters.The optimal temperature for growth of W2,Y1 and X was 30℃,35℃,and 30℃ respectively.The optimal range of pH for W2 was 6.0~9.0,and that for Y1 and Xwas 6.0~8.0;the optimal initial concentration for clomazone of W2 was 200 mg a.i.·L-1,and that for Y1 and X was 100 mg a.i.·L-1.
【Key words】 Clomazone; Degrading microorganisms; Enrichment and isolation; Identification;
- 【文献出处】 中国油料作物学报 ,Chinese Journal of Oil Crop Sciences , 编辑部邮箱 ,2007年03期
- 【分类号】X173
- 【被引频次】13
- 【下载频次】176