【摘要】 目的探讨幽门螺杆菌(Hp)、端粒酶基因(hTERT)、p53在胃癌及癌前病变中的表达及相关性。方法采用特殊染色、免疫组化和原位杂交方法,分别检测130例石蜡包埋标本中的Hp感染、p53蛋白和hTERT mRNA的表达。结果①在慢性浅表性胃炎、慢性萎缩性胃炎、非典型增生和胃癌患者中Hp的感染率分别为40%、60%、70%和72.5%。胃癌与慢性浅表性胃炎比较差异有统计学意义(P<0.05)。四种病变中p53蛋白的表达率分别为5%、25%、50%、62.5%,其中胃癌与慢性浅表性胃炎、慢性萎缩性胃炎比较差异有统计学意义(P<0.01)。hTERT mRNA的表达率分别为0、10%、30%、78.75%,胃癌与慢性萎缩性胃炎、非典型增生相比差异有统计学意义(P<0.05)。②Hp感染阳性的胃癌病例,p53、hTERT mRNA阳性的表达率明显高于Hp阴性胃癌病例(P<0.05);p53阳性的胃癌中端粒酶基因表达均为阳性,p53阴性的胃癌中有部分端粒酶基因表达阳性(66.7%)。结论Hp感染与p53基因的突变、hTERT活性密切相关;p53基因突变可导致端粒酶活化,但端粒酶激活可能不完全依赖于p53基因的调控。更多还原

【Abstract】 Objective To study the expression of Hp,hTERT,p53 and their relations in gastric cancer and precancerous lesions.Methods Special staining,immunohis to chemistry and in situ hybridization were used to de- tect Hp infection,the expression of p53 protein and hTERT mRNA in 130 cases of paraffin imbedding specimen of different gastric mucosal lesions.Results①In chronic superficial gastritis(GSG),chronic strophic gastritis (CAG),dysplasia(DYS)and gastric carcinoma(GC),the expression rates of Hp infection were 40%,60%,70%, and 72.5% respectively.There were significant differences between GC and CSG(P<0.05).The expression rates of p53 protein were 5%,25%,50% and 62.5% respectively;there were significant differences between GC and CAG,GC and CSG(P<0.01).The expression rates of hTERT mRNA were 0%,10%,30% and 78.75% respec- tively,there were significant differences between GC and CAG,GC and CSG,GC and DYS(P<0.05).②The p53, hTERT positive expression rate in gastric cancer was much higher in the Hp positive group than in the negative one (P<0.05);The expression of hTERTmRNA was positive in all the p53 positive gastric cancer cases,while it was partly positive(66.7%)in the p53 negative cases.Conclusion The mutation of p53,hTERT activation are closely associated with Hp infection;The mutation of p53 can induce the activation of telomerase,but the activation of te- lomerase does not depend on the p53 gene completely.更多还原